Standard Curve (Sample)
(Fig.1. Human Ubiquilin-2 (UBQLN2) Standard Curve.)
Human Ubiquilin-2 (UBQLN2) ELISA Kit | UBQLN2 elisa kit
Human Ubiquilin-2 (UBQLN2) ELISA Kit
Gene Names
UBQLN2; DSK2; ALS15; CHAP1; N4BP4; PLIC2; HRIHFB2157
Reactivity
Human
Synonyms
Ubiquilin-2 (UBQLN2); N/A; Human Ubiquilin-2 (UBQLN2) ELISA Kit; UBQLN2; CHAP1; DSK2; FLJ10167; FLJ56541; HRIHFB2157; N4BP4; PLIC2; Nedd4 binding protein 4; protein linking IAP with cytoskeleton 2; ubiquitin-like product Chap1/Dsk2; UBQLN2 elisa kit
Reactivity
Human
Specificity
Human Ubiquilin-2 (UBQLN2) ELISA Kit has high sensitivity and excellent specificity for detection of Human UBQLN2. No significant cross-reactivity or interference between Human UBQLN2 and analogues was observed.
Sequence Length
624
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Ubiquilin-2 (UBQLN2) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Ubiquilin-2 (UBQLN2) Standard Curve.)
Related Product Information for UBQLN2 elisa kit
Description: This Human Ubiquilin-2 (UBQLN2) ELISA Kit employs a two-site sandwich ELISA to quantitate UBQLN2 in samples. An antibody specific for UBQLN2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any UBQLN2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UBQLN2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UBQLN2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Ubiquilin-2 is a ubiquitin-like protein (ubiquilin) that shares high degree of similarity with related products in yeast, rat and frog. Ubiquilins contain a N-terminal ubiquitin-like domain and a C-terminal ubiquitin-associated domain. They physically associate with both proteasomes and ubiquitin ligases, and thus thought to functionally link the ubiquitination machinery to the proteasome to affect in vivo protein degradation. This ubiquilin has also been shown to bind the ATPase domain of the Hsp70-like Stch protein. The predicted 624-amino acid PLIC2 protein, also called UBQLN2, shares 72% amino acid identity with PLIC1. Two motifs are conserved in the mammalian PLICs and yeast Dsk2, an N-terminal ubiquitin-like (UBL) domain and a C-terminal ubiquitin-associated (UBA) domain.
Background: Ubiquilin-2 is a ubiquitin-like protein (ubiquilin) that shares high degree of similarity with related products in yeast, rat and frog. Ubiquilins contain a N-terminal ubiquitin-like domain and a C-terminal ubiquitin-associated domain. They physically associate with both proteasomes and ubiquitin ligases, and thus thought to functionally link the ubiquitination machinery to the proteasome to affect in vivo protein degradation. This ubiquilin has also been shown to bind the ATPase domain of the Hsp70-like Stch protein. The predicted 624-amino acid PLIC2 protein, also called UBQLN2, shares 72% amino acid identity with PLIC1. Two motifs are conserved in the mammalian PLICs and yeast Dsk2, an N-terminal ubiquitin-like (UBL) domain and a C-terminal ubiquitin-associated (UBA) domain.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
ubiquilin-2
NCBI Official Synonym Full Names
ubiquilin 2
NCBI Official Symbol
UBQLN2
NCBI Official Synonym Symbols
DSK2; ALS15; CHAP1; N4BP4; PLIC2; HRIHFB2157
NCBI Protein Information
ubiquilin-2
UniProt Protein Name
Ubiquilin-2
UniProt Gene Name
UBQLN2
UniProt Synonym Gene Names
N4BP4; PLIC2; PLIC-2; hPLIC-2
UniProt Entry Name
UBQL2_HUMAN
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Product Notes
The Human UBQLN2 ubqln2 (Catalog #AAA330320) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330320 ELISA Kit recognizes Human UBQLN2. It is sometimes possible for the material contained within the vial of "Ubiquilin-2 (UBQLN2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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