Human Urea ELISA Kit | ureA elisa kit
Human Urea ELISA Kit
Reactivity
Human
Synonyms
Urea; N/A; Human Urea ELISA Kit; ureA elisa kit
Reactivity
Human
Sequence Length
100
Assay Type
Sandwich
Detection Range
200 mmol/L-3.12 mmol/L
Sensitivity
Up to 0.6 mmol/L
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for ureA elisa kit
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Human Urea monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
11,128 Da
NCBI Official Full Name
UreA
UniProt Protein Name
Urease subunit gamma
UniProt Gene Name
ureA
UniProt Synonym Gene Names
R02475; SMc01941
UniProt Entry Name
URE3_RHIME
