Standard Curve (Sample)
(Fig.1. Human Vacuolar protein sorting-associated protein 37A (VPS37A) Standard Curve.)
Human Vacuolar Protein Sorting-Associated Protein 37A (VPS37A) ELISA Kit | VPS37A elisa kit
Human Vacuolar Protein Sorting-Associated Protein 37A (VPS37A) ELISA Kit
Gene Names
VPS37A; HCRP1; PQBP2; SPG53
Reactivity
Human
Synonyms
Vacuolar Protein Sorting-Associated Protein 37A (VPS37A); N/A; Human Vacuolar Protein Sorting-Associated Protein 37A (VPS37A) ELISA Kit; VPS37A; FLJ32642; FLJ42616; HCRP1; PQBP2; hepatocellular carcinoma related protein 1; polyglutamine binding protein 2; vacuolar protein sorting 37A; VPS37A elisa kit
Reactivity
Human
Specificity
Human Vacuolar protein sorting-associated protein 37A (VPS37A) ELISA Kit has high sensitivity and excellent specificity for detection of Human VPS37A. No significant cross-reactivity or interference between Human VPS37A and analogues was observed.
Sequence Length
397
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Vacuolar protein sorting-associated protein 37A (VPS37A) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Vacuolar protein sorting-associated protein 37A (VPS37A) Standard Curve.)
Related Product Information for VPS37A elisa kit
Description: This Human Vacuolar protein sorting-associated protein 37A (VPS37A) ELISA Kit employs a two-site sandwich ELISA to quantitate VPS37A in samples. An antibody specific for VPS37A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VPS37A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VPS37A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VPS37A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: HCRP1 encodes a deduced 397-amino acid protein. Northern blot analysis showed ubiquitous expression of an approximately 2-kb HCRP1 transcript, with most abundant expression in liver. Western blot analysis showed reduced HCRP1 expression in 6 of 8 HCC tissues, but higher expression levels of HCRP1 in adjacent normal tissues in all 8 cases. Transient transfection of GFP-HCRP1 fusion constructs showed that HCRP1 is localized throughout the cell, but predominantly in the nucleus.Pull-down assays demonstrated that the interaction between HCRP1 and TSG101 occurs through the mod(r) domain. By size exclusion chromatography, HCRP1 cofractionated with TSG101 and VPS28. Depletion of TSG101 by siRNA treatment resulted in reduction in HCRP1 levels in HeLa cells.
Background: HCRP1 encodes a deduced 397-amino acid protein. Northern blot analysis showed ubiquitous expression of an approximately 2-kb HCRP1 transcript, with most abundant expression in liver. Western blot analysis showed reduced HCRP1 expression in 6 of 8 HCC tissues, but higher expression levels of HCRP1 in adjacent normal tissues in all 8 cases. Transient transfection of GFP-HCRP1 fusion constructs showed that HCRP1 is localized throughout the cell, but predominantly in the nucleus.Pull-down assays demonstrated that the interaction between HCRP1 and TSG101 occurs through the mod(r) domain. By size exclusion chromatography, HCRP1 cofractionated with TSG101 and VPS28. Depletion of TSG101 by siRNA treatment resulted in reduction in HCRP1 levels in HeLa cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Vacuolar protein sorting-associated protein 37A
NCBI Official Synonym Full Names
VPS37A subunit of ESCRT-I
NCBI Official Symbol
VPS37A
NCBI Official Synonym Symbols
HCRP1; PQBP2; SPG53
NCBI Protein Information
vacuolar protein sorting-associated protein 37A
UniProt Protein Name
Vacuolar protein sorting-associated protein 37A
UniProt Gene Name
VPS37A
UniProt Synonym Gene Names
HCRP1; hVps37A
UniProt Entry Name
VP37A_HUMAN
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Product Notes
The Human VPS37A vps37a (Catalog #AAA330275) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330275 ELISA Kit recognizes Human VPS37A. It is sometimes possible for the material contained within the vial of "Vacuolar Protein Sorting-Associated Protein 37A (VPS37A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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