Human Vascular Endothelial Cell Growth Factor B (VEGF-B) ELISA Kit | VEGF-B elisa kit

Human Vascular Endothelial Cell Growth Factor B (VEGF-B) ELISA Kit

Cat. Num. AAA332004

• Gene Names: VEGFB; VRF; VEGFL
• Reactivity: Human
• Synonyms: Vascular Endothelial Cell Growth Factor B (VEGF-B); N/A; Human Vascular Endothelial Cell Growth Factor B (VEGF-B) ELISA Kit; VEGFB; VEGFL; VRF; VEGF-B elisa kit

• Reactivity: Human
• Specificity: Human Vascular Endothelial cell Growth Factor B (VEGF-B) ELISA Kit has high sensitivity and excellent specificity for detection of Human VEGFB. No significant cross-reactivity or interference between Human VEGFB and analogues was observed.
• Sequence Length: 188
• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids

• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Standard Curve (Sample)

(Fig.1. Human Vascular Endothelial cell Growth Factor B (VEGF-B) Standard Curve.)

Related Product Information for VEGF-B elisa kit

Description: This Human Vascular Endothelial cell Growth Factor B (VEGF-B) ELISA Kit employs a two-site sandwich ELISA to quantitate VEGFB in samples. An antibody specific for VEGFB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VEGFB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VEGFB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGFB bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Vascular endothelial growth factor B is a growth factor that belongs to the vascular endothelial growth factor family of which VEGF-A is the best known member. In contrast to VEGF-A, VEGF-B plays a less pronounced role in the vascular system: whereas VEGF-A is important for the formation of blood vessels, such as during development or in pathological conditions, VEGF-B seems only to play in role in the maintenance of newly formed blood vessels during pathological conditions. VEGF-B plays also an important role on several types of neurons. It is important for the protection of neurons in the retina and the cerebral cortex during stroke and of motoneurons during motor neuron diseases such as amyotrophic lateral sclerosis.VEGF-B exerts its effects via the FLT1 receptor.

NCBI and Uniprot Product Information

• NCBI GI #: 344179103
• NCBI GeneID: 7423
• NCBI Accession #: NP_001230662.1
• NCBI GenBank Nucleotide #: NP_001230662.1
• UniProt Accession #: P49765
• NCBI Official Full Name: vascular endothelial growth factor B isoform VEGFB-167
• NCBI Official Synonym Full Names: vascular endothelial growth factor B
• NCBI Official Symbol: VEGFB
• NCBI Official Synonym Symbols: VRF; VEGFL
• NCBI Protein Information: vascular endothelial growth factor B
• UniProt Protein Name: Vascular endothelial growth factor B
• UniProt Gene Name: VEGFB
• UniProt Synonym Gene Names: VRF; VEGF-B; VRF
• UniProt Entry Name: VEGFB_HUMAN