Human Vitamin D Receptor (VDR) ELISA Kit | VDR elisa kit

Human Vitamin D Receptor (VDR) ELISA Kit

Cat. Num. AAA67495

• Gene Names: VDR; NR1I1; PPP1R163
• Reactivity: Human
• Synonyms: Vitamin D Receptor (VDR); N/A; Human Vitamin D Receptor (VDR) ELISA Kit; NR1I1 Nuclear Receptor Subfamily 1 Group I Member 1 Calcitriol Receptor 1,25-dihydroxyvitamin D3 receptor; VDR elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of VDR. No significant cross-reactivity or interference between VDR and analogues was observed.
• Sequence Length: 427
• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.625-40ng/mL
• Sensitivity: 0.224ng/mL

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level VDR were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level VDR were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Standard Curve (Sample)

Related Product Information for VDR elisa kit

Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of VDR in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to VDR. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to VDR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain VDR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of VDR in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4507883
• NCBI GeneID: 7421
• NCBI Accession #: NP_000367.1
• NCBI GenBank Nucleotide #: NM_000376.2
• UniProt Accession #: P11473; Q5PSV3; B2R5Q1; G3V1V9
• Molecular Weight: 53,883 Da
• NCBI Official Full Name: vitamin D3 receptor isoform VDRA
• NCBI Official Synonym Full Names: vitamin D (1,25- dihydroxyvitamin D3) receptor
• NCBI Official Symbol: VDR
• NCBI Official Synonym Symbols: NR1I1; PPP1R163
• NCBI Protein Information: vitamin D3 receptor
• UniProt Protein Name: Vitamin D3 receptor
• UniProt Gene Name: VDR
• UniProt Synonym Gene Names: NR1I1; VDR