Standard Curve (Sample)
(Fig.1. Human WAP four-disulfide core domain protein 5 (WFDC5) Standard Curve.)
Human WAP Four-Disulfide Core Domain Protein 5 (WFDC5) ELISA Kit | WFDC5 elisa kit
Human WAP Four-Disulfide Core Domain Protein 5 (WFDC5) ELISA Kit
Gene Names
WFDC5; PRG5; WAP1; dJ211D12.5
Reactivity
Human
Synonyms
WAP Four-Disulfide Core Domain Protein 5 (WFDC5); N/A; Human WAP Four-Disulfide Core Domain Protein 5 (WFDC5) ELISA Kit; WFDC5; PRG5; WAP1; dJ211D12.5; p53-responsive gene 5; protease inhibitor WAP1; WFDC5 elisa kit
Reactivity
Human
Specificity
Human WAP four-disulfide core domain protein 5 (WFDC5) ELISA Kit has high sensitivity and excellent specificity for detection of Human WFDC5. No significant cross-reactivity or interference between Human WFDC5 and analogues was observed.
Sequence Length
123
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human WAP four-disulfide core domain protein 5 (WFDC5) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human WAP four-disulfide core domain protein 5 (WFDC5) Standard Curve.)
Related Product Information for WFDC5 elisa kit
Description: This Human WAP four-disulfide core domain protein 5 (WFDC5) ELISA Kit employs a two-site sandwich ELISA to quantitate WFDC5 in samples. An antibody specific for WFDC5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WFDC5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WFDC5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WFDC5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: WFDC5 encodes a member of the WAP-type four-disulfide core (WFDC) domain family. Most WFDC proteins contain only one WFDC domain, and this encoded protein contains two WFDC domains. The WFDC domain, or WAP signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. The deduced 123-amino acid protein contains an N-terminal signal peptide, followed by 2 WAP domains. Each WAP domain contains 8 cysteines that form conserved disulfide bridges, and each WAP domain forms a proposed protease-binding loop and a beta sheet. The mature 99-amino acid WAP1 protein has a calculated molecular mass of 10.9 kD.
Background: WFDC5 encodes a member of the WAP-type four-disulfide core (WFDC) domain family. Most WFDC proteins contain only one WFDC domain, and this encoded protein contains two WFDC domains. The WFDC domain, or WAP signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. The deduced 123-amino acid protein contains an N-terminal signal peptide, followed by 2 WAP domains. Each WAP domain contains 8 cysteines that form conserved disulfide bridges, and each WAP domain forms a proposed protease-binding loop and a beta sheet. The mature 99-amino acid WAP1 protein has a calculated molecular mass of 10.9 kD.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
WAP four-disulfide core domain protein 5
NCBI Official Synonym Full Names
WAP four-disulfide core domain 5
NCBI Official Symbol
WFDC5
NCBI Official Synonym Symbols
PRG5; WAP1; dJ211D12.5
NCBI Protein Information
WAP four-disulfide core domain protein 5
UniProt Protein Name
WAP four-disulfide core domain protein 5
UniProt Gene Name
WFDC5
UniProt Synonym Gene Names
PRG5; WAP1
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Product Notes
The Human WFDC5 wfdc5 (Catalog #AAA330262) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330262 ELISA Kit recognizes Human WFDC5. It is sometimes possible for the material contained within the vial of "WAP Four-Disulfide Core Domain Protein 5 (WFDC5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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