Standard Curve (Sample)
Human XCL1/Lymphotactin ELISA Kit | XCL1 elisa kit
Human XCL1/Lymphotactin PicoKine ELISA Kit
Gene Names
XCL1; LTN; ATAC; LPTN; SCM1; SCM-1; SCM1A; SCYC1; SCM-1a
Reactivity
Human
Synonyms
XCL1/Lymphotactin; N/A; Human XCL1/Lymphotactin PicoKine ELISA Kit; Lymphotactin; chemokine (C motif) ligand 1; ATAC; C motif chemokine 1; Chemokine (C motif) ligand 1; Cytokine SCM-1; Cytokine SCM1; LPTN; LTN; Lymphotaxin; SCM 1; SCM 1 alpha; SCM-1-alpha; SCM1; SCM1 alpha; SCYC1; Small inducible cytokine C1; Small inducible cytokine subfamily C member 1; Small-inducible cytokine C1; XC chemokine ligand 1; Xcl1; XCL1_HUMAN; XCL1 elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human XCL1
Sequence Length
114
Samples
Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Detection Range
62.5 pg/ml - 4,000 pg/ml
Sensitivity
<1 pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for XCL1 elisa kit
Background: Chemokine (C motif) ligand (XCL1) is a small cytokine belonging to the XC chemokine family that is also known as lymphotactin. This gene is mapped to chromosome 1q23 by FISH. The sequence of the deduced 114-amino acid protein is most homologous to the CC chemokines CCL8 and CCL3. It is found in high levels in spleen, thymus, intestine and peripheral blood leukocytes, and at lower levels in lung, prostate gland and ovary. XCL1 induces the migration of cells expressing XCRR1.
Principle of the Assay: The Picokine™ Human XCL1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human XCL1 with a 96-well strip plate that is pre-coated with antibody specific for XCL1. The detection antibody is a biotinylated antibody specific for XCL1. The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human XCL1 with immunogen: Expression system for standard: E.coli; Immunogen sequence: V22-G114. The kit is analytically validated with ready-to-use reagents. To measure Human XCL1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human XCL1 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human XCL1 in the sample.
Principle of the Assay: The Picokine™ Human XCL1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human XCL1 with a 96-well strip plate that is pre-coated with antibody specific for XCL1. The detection antibody is a biotinylated antibody specific for XCL1. The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human XCL1 with immunogen: Expression system for standard: E.coli; Immunogen sequence: V22-G114. The kit is analytically validated with ready-to-use reagents. To measure Human XCL1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human XCL1 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human XCL1 in the sample.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
12,517 Da
NCBI Official Full Name
Lymphotactin
NCBI Official Synonym Full Names
chemokine (C motif) ligand 1
NCBI Official Symbol
XCL1
NCBI Official Synonym Symbols
LTN; ATAC; LPTN; SCM1; SCM-1; SCM1A; SCYC1; SCM-1a
NCBI Protein Information
lymphotactin; SCM-1-alpha; lymphotaxin; cytokine SCM-1; c motif chemokine 1; XC chemokine ligand 1; single cysteine motif 1a; small-inducible cytokine C1; small inducible cytokine subfamily C, member 1 (lymphotactin)
UniProt Protein Name
Lymphotactin
UniProt Gene Name
XCL1
UniProt Synonym Gene Names
LTN; SCYC1
UniProt Entry Name
XCL1_HUMAN
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Product Notes
The Human XCL1 xcl1 (Catalog #AAA11612) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA11612 ELISA Kit recognizes Human XCL1. It is sometimes possible for the material contained within the vial of "XCL1/Lymphotactin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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