Application Data
(This assay recognizes both recombinant and natural Human XPNPEP2. The factors listed below were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.)
Human XPNPEP2 ELISA Kit | XPNPEP2 elisa kit
Human XPNPEP2 ELISA Kit
Reactivity
Human
Synonyms
XPNPEP2; N/A; Human XPNPEP2 ELISA Kit; XPNPEP2 ELISA Kit, Human; X-prolyl aminopeptidase (aminopeptidase P) 2, membrane-bound; AEACEI ELISA Kit, Human; APP2 ELISA Kit, Human; XPNPEP2 elisa kit
Reactivity
Human
Specificity
For the quantitative determination of Human XPNPEP2 concentration in serum.
Assay Type
Solid Phase Sandwich ELISA (quantitative)
Sample Types
Serum
Detection Range
9.38-600pg/mL
Sensitivity
2.10pg/mL
Conjugation
HRP
Materials Provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Application Data
(This assay recognizes both recombinant and natural Human XPNPEP2. The factors listed below were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.)
Application Data
(This assay recognizes both recombinant and natural Human XPNPEP2. The factors listed below were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.)
Standard Curve (Sample)
(This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.)
Standard Curve (Sample)
(This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.)
Related Product Information for XPNPEP2 elisa kit
This XPNPEP2 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human XPNPEP2 protein in Serum. It contains recombinant Human XPNPEP2, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Background: Aminopeptidase P (APP) is a hydrolase specific for N-terminal imido bonds, which are common to several collagen degradation products, neuropeptides, vasoactive peptides, and cytokines. A membrane-bound and soluble form of this enzyme (XPNPEP2) have been identified as products of two separate genes. XPNPEP2, the X-linked gene that encodes membranous aminopeptidase P (APP), has been reported to associate with APP activity. The membrane aminopeptidase P (XPNPEP2) is largely limited in distribution to endothelia and brush border epithelia. APP and XPNPEP2 contain homologous blocks of sequence common to members of the "pita bread-fold" protein family, of which Escherichia coli methionine aminopeptidase is the prototype. The C-2399A variant in XPNPEP2 is associated with reduced APP activity and a higher incidence of AE-ACEi. XPNPEP2 mRNA was detected in fibroblasts that carry the translocation, suggesting that this gene at least partially escapes X inactivation. XPNPEP2 is a candidate gene for premature ovarian failure (POF).
Background: Aminopeptidase P (APP) is a hydrolase specific for N-terminal imido bonds, which are common to several collagen degradation products, neuropeptides, vasoactive peptides, and cytokines. A membrane-bound and soluble form of this enzyme (XPNPEP2) have been identified as products of two separate genes. XPNPEP2, the X-linked gene that encodes membranous aminopeptidase P (APP), has been reported to associate with APP activity. The membrane aminopeptidase P (XPNPEP2) is largely limited in distribution to endothelia and brush border epithelia. APP and XPNPEP2 contain homologous blocks of sequence common to members of the "pita bread-fold" protein family, of which Escherichia coli methionine aminopeptidase is the prototype. The C-2399A variant in XPNPEP2 is associated with reduced APP activity and a higher incidence of AE-ACEi. XPNPEP2 mRNA was detected in fibroblasts that carry the translocation, suggesting that this gene at least partially escapes X inactivation. XPNPEP2 is a candidate gene for premature ovarian failure (POF).
References
Sprinkle TJ, et al. (2000) Cloning, chromosomal sublocalization of the human soluble aminopeptidase P gene (XPNPEP1) to 10q25.3 and conservation of the putative proton shuttle and metal ligand binding sites with XPNPEP2. Arch Biochem Biophys. 378(1): 51-6.
Prueitt RL, et al. (2000) Physical mapping of nine Xq translocation breakpoints and identification of XPNPEP2 as a premature ovarian failure candidate gene. Cytogenet Cell Genet. 89(1-2): 44-50.
Duan QL, et al. (2005) A variant in XPNPEP2 is associated with angioedema induced by angiotensin I-converting enzyme inhibitors. Am J Hum Genet. 77(4): 617-26.
Woodard-Grice AV, et al. (2010) Sex-dependent and race-dependent association of XPNPEP2 C-2399A polymorphism with angiotensin-converting enzyme inhibitor-associated angioedema. Pharmacogenet Genomics. 20(9): 532-6.
Prueitt RL, et al. (2000) Physical mapping of nine Xq translocation breakpoints and identification of XPNPEP2 as a premature ovarian failure candidate gene. Cytogenet Cell Genet. 89(1-2): 44-50.
Duan QL, et al. (2005) A variant in XPNPEP2 is associated with angioedema induced by angiotensin I-converting enzyme inhibitors. Am J Hum Genet. 77(4): 617-26.
Woodard-Grice AV, et al. (2010) Sex-dependent and race-dependent association of XPNPEP2 C-2399A polymorphism with angiotensin-converting enzyme inhibitor-associated angioedema. Pharmacogenet Genomics. 20(9): 532-6.
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Product Notes
The Human XPNPEP2 (Catalog #AAA258440) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA258440 ELISA Kit recognizes Human XPNPEP2. It is sometimes possible for the material contained within the vial of "XPNPEP2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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