Human, Mouse Cortisol ELISA Kit

Multi-Species Cortisol ELISA Kit

Cat. Num. AAA266532

• Reactivity: Human, Mouse
• Synonyms: Cortisol; N/A; Multi-Species Cortisol ELISA Kit; Cortisol elisa kit

• Reactivity: Human, Mouse
• Samples: dried fecal extracts, saliva, urine, serum, plasma and tissue culture media samples.
• Detection Range: 8.5 to 23.8 ug/dL
• Sensitivity: 27.6pg/mL
• Detection Target: Cortisol

• Preparation and Storage: Store at 2-8 degree C

Standard Curve (Sample)

Related Product Information for Cortisol elisa kit

Background: Cortisol, C21H30O5, (hydrocortisone, compound F) is the primary glucocorticoid produced and secreted by the adrenal cortex. It is often referred to as the "stress hormone" as it is involved in the response to stress and it affects blood pressure, blood sugar levels, and other actions of stress adaptation. Immunologically, cortisol functions as an important anti-inflammatory and plays a role in hypersensitivity, immunosuppression, and disease resistance1. In the metabolic aspect, cortisol promotes gluconeogenesis, liver glycogen deposition, and the reduction of glucose utilization2. Production of cortisol follows an ACTH-dependent circadian rhythm, with a peak level in the morning and decreasing levels throughout the day. Most serum cortisol, all but about 4%, is bound to proteins including corticosteroid binding globulin and serum albumin1,3. Only free cortisol is available to most receptors and it is through these receptors that physiological processes are modulated. Abnormal cortisol levels are being evaluated for correlation with a variety of different conditions, such as prostate cancer4, depression5, and schizophrenia6. It is already known that abnormal levels of cortisol are involved in Cushing's Syndrome and Addision's disease7.

Principle of the Assay: The Multi-Species Cortisol ELISA Kitis designed to quantitatively measure cortisol present in dried fecal extracts, saliva, urine, serum, plasma and tissue culture media samples. Please read the complete kit insert before performing this assay. Total cortisol is measured in extracted samples and in serum and plasma and free cortisol in saliva and urine. A cortisol standard is provided to generate a standard curve for the assay and all samples must be read off a user-generated standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture mouse antibodies. A cortisol- peroxidase conjugate is added to the wells. The binding reaction is initiated by the addition of a monoclonal antibody to cortisol. The immunological reaction occurs between the limiting amount of added anti-cortisol monoclonal antibody, the cortisol antigen in the sample or standard, and the limiting amount of added cortisol-peroxidase conjugate. As the concentration of cortisol in the sample increases, the amount of cortisol-peroxidase conjugate bound decreases causing an decrease in signal, and vice versa. The signal is generated from the cortisol-peroxidase bound to the anticortisol antibody which itself is bound to the goat anti-mouse IgG coated plates. Excess cortisol-peroxidase does not bind to the plates and is washed out of the well prior to the addition of substrate. After an hour incubation the plate is washed, and substrate is added. The substrate reacts with the bound cortisolperoxidase conjugate. After a short incubation, the reaction is stopped, and the intensity of the generated color is detected in a microtiter plate reader at 450 nm wavelength. The concentration of the cortisol in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers.