IF (Immunofluorescence)
(PMA +- Immunofluorescence analysis of HeLa cells, treated with PMA (125ng/ml, 30mins), using 14-3-3 zeta (phospho-Ser58) antibody.)
14-3-3 zeta Antibody | anti-14-3-3 zeta antibody
14-3-3 zeta (Phospho-Ser58) Antibody
Gene Names
YWHAZ; HEL4; YWHAD; KCIP-1; HEL-S-3; HEL-S-93; 14-3-3-zeta
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
14-3-3 zeta; N/A; 14-3-3 zeta (Phospho-Ser58) Antibody; 14-3-3 protein zeta/delta; 1433Z; 143Z; FAS; Factor activating exoenzyme S; KCIP-1; Mitochondrial import stimulation factor S1 subunit; Protein kinase C inhibitor protein-1; YWHAZ; anti-14-3-3 zeta antibody
Reactivity
Human, Mouse, Rat
Specificity
14-3-3 zeta (phospho-Ser58) antibody detects endogenous levels of 14-3-3 zeta only when phosphorylated at serine 58.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
245
Applicable Applications for anti-14-3-3 zeta antibody
ELISA, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human 14-3-3 zeta around the phosphorylation site of serine 58 (R-S-SP-W-R).
All Sites
Human: Ser58
Mouse: Ser58
Rat: Ser58
Mouse: Ser58
Rat: Ser58
Preparation and Storage
Store at-20 degree C for 1 year
IF (Immunofluorescence)
(PMA +- Immunofluorescence analysis of HeLa cells, treated with PMA (125ng/ml, 30mins), using 14-3-3 zeta (phospho-Ser58) antibody.)
IF (Immunofluorescence)
(PMA +- Immunofluorescence analysis of HeLa cells, treated with PMA (125ng/ml, 30mins), using 14-3-3 zeta (phospho-Ser58) antibody.)
WB (Western Blot)
(Western blot analysis of extracts from NIH/3T3 cells treated with UV (30mins), using 14-3-3 zeta (phospho-Ser58) antibody.)
WB (Western Blot)
(Western blot analysis of extracts from NIH/3T3 cells treated with UV (30mins), using 14-3-3 zeta (phospho-Ser58) antibody.)
IHC (Immunohistochemistry)
(P-peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 14-3-3 zeta (phospho-Ser58) antibody.)
IHC (Immunohistochemistry)
(P-peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 14-3-3 zeta (phospho-Ser58) antibody.)
Product Categories/Family for anti-14-3-3 zeta antibody
References
Ying H. Shen Mol. Biol. Cell, Nov 2003; 14: 4721. David W. Powell, Mol. Cell. Biol., Aug 2003; 23: 5376-5387. Mackintosh. C, (2004) Biochem. J. 381, 329-42. Dougherty, M.K. and Morrison, D.K. (2004) J. Cell Sci. 117, 1875-84.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
19,333 Da
NCBI Official Full Name
14-3-3 protein zeta/delta
NCBI Official Synonym Full Names
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta
NCBI Official Symbol
YWHAZ
NCBI Official Synonym Symbols
HEL4; YWHAD; KCIP-1; HEL-S-3; HEL-S-93; 14-3-3-zeta
NCBI Protein Information
14-3-3 protein zeta/delta
UniProt Protein Name
14-3-3 protein zeta/delta
UniProt Gene Name
YWHAZ
UniProt Synonym Gene Names
KCIP-1
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Product Notes
The 14-3-3 zeta ywhaz (Catalog #AAA108930) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The 14-3-3 zeta (Phospho-Ser58) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's 14-3-3 zeta can be used in a range of immunoassay formats including, but not limited to, ELISA, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the 14-3-3 zeta ywhaz for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "14-3-3 zeta, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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