WB (Western Blot)
(Western blot analysis of extracts from HepG2 cells treated with EGF (200ng/ml, 5mins), using C/EBP-alpha (phospho-Ser21) antibody.)
C/EBP-alpha Antibody | anti-C/EBP-alpha antibody
C/EBP-alpha (Phospho-Ser21) Antibody
Gene Names
CEBPA; CEBP; C/EBP-alpha
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
C/EBP-alpha; N/A; C/EBP-alpha (Phospho-Ser21) Antibody; Apoptotic cysteine protease; Apoptotic protease Mch-5; CAP4; Caspase-8 precursor; EC 3.4.22.-; FADD-homologous ICE/CED-3-like protease; FADD-like ICE; FLICE; ICE-like apoptotic protease 5; ICE8; MACH; MCH5; MORT1-associated CED-3 homolog; anti-C/EBP-alpha antibody
Reactivity
Human, Mouse, Rat
Specificity
C/EBP-alpha (phospho-Ser21) antibody detects endogenous levels of C/EBP-alpha only when phosphorylated at serine 21.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
239
Applicable Applications for anti-C/EBP-alpha antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human C/EBP-alpha around the phosphorylation site of serine 21 (L-Q-SP-P-P).
All Sites
Human: Ser21
Mouse: Ser21
Rat: Ser21
Mouse: Ser21
Rat: Ser21
Preparation and Storage
Store at-20 degree C for 1 year
WB (Western Blot)
(Western blot analysis of extracts from HepG2 cells treated with EGF (200ng/ml, 5mins), using C/EBP-alpha (phospho-Ser21) antibody.)
WB (Western Blot)
(Western blot analysis of extracts from HepG2 cells treated with EGF (200ng/ml, 5mins), using C/EBP-alpha (phospho-Ser21) antibody.)
IHC (Immunohistochemistry)
(P-peptide-+ Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using C/EBP-alpha (phospho-Ser21) antibody.)
IHC (Immunohistochemistry)
(P-peptide-+ Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using C/EBP-alpha (phospho-Ser21) antibody.)
Product Categories/Family for anti-C/EBP-alpha antibody
References
Takashi Akasaka, Blood, Apr 2007; 109: 3451-3461. Carolina Gillio-Meina, Biol Reprod, May 2005; 72: 1194-1204. Raphaël Métivier, Mol. Endocrinol., Nov 2001; 15: 1953. Neoncheol Jung, Eur. J. Biochem., Oct 2000; 267: 6180.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
41,249 Da
NCBI Official Full Name
CCAAT/enhancer-binding protein alpha isoform b
NCBI Official Synonym Full Names
CCAAT enhancer binding protein alpha
NCBI Official Symbol
CEBPA
NCBI Official Synonym Symbols
CEBP; C/EBP-alpha
NCBI Protein Information
CCAAT/enhancer-binding protein alpha
UniProt Protein Name
CCAAT/enhancer-binding protein alpha
UniProt Gene Name
CEBPA
UniProt Synonym Gene Names
C/EBP alpha
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Product Notes
The C/EBP-alpha cebpa (Catalog #AAA109247) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The C/EBP-alpha (Phospho-Ser21) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's C/EBP-alpha can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the C/EBP-alpha cebpa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "C/EBP-alpha, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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