IF (Immunofluorescence)
(P-peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using NF-kappaB p65 (Phospho-Ser276) antibody.)
NF-kappaB p65 Antibody | anti-RELA antibody
NF-kappaB p65 (Phospho-Ser276) Antibody
Gene Names
RELA; p65; NFKB3
Reactivity
Human, Mouse, Rat
Applications
Immunoprecipitation, ELISA, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
NF-kappaB p65; N/A; NF-kappaB p65 (Phospho-Ser276) Antibody; anti-RELA antibody
Reactivity
Human, Mouse, Rat
Specificity
NF-kappaB p65 (Phospho-Ser276) Antibody detects endogenous levels of NF-kappaB p65 only when phosphorylated at serine 276.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
548
Applicable Applications for anti-RELA antibody
IP (Immunoprecipitation), ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human NF-kappaB p65 around the phosphorylation site of serine 276.
Preparation and Storage
Store at-20 degree C for 1 year
IF (Immunofluorescence)
(P-peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using NF-kappaB p65 (Phospho-Ser276) antibody.)
IF (Immunofluorescence)
(P-peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using NF-kappaB p65 (Phospho-Ser276) antibody.)
WB (Western Blot)
(Western blot analysis of extracts from JurKat cells, using NF-kappaB p65 (Phospho-Ser276) antibody. Western blot analysis of extracts from HeLa cells and 3T3 cells, using NF-kappaB p65 (Phospho-Ser276) antibody.)
WB (Western Blot)
(Western blot analysis of extracts from JurKat cells, using NF-kappaB p65 (Phospho-Ser276) antibody. Western blot analysis of extracts from HeLa cells and 3T3 cells, using NF-kappaB p65 (Phospho-Ser276) antibody.)
Application Data
Application Data
Product Categories/Family for anti-RELA antibody
References
Baeuerle P A, et al. (1994) Annu Rev Immunol. 12:141-179. Baeuerle P A, et al. (1996) Cell 87:13-20. Haskill S, et al. (1991) Cell 65:1281-1289.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
59,910 Da
NCBI Official Full Name
transcription factor p65 isoform 2
NCBI Official Synonym Full Names
RELA proto-oncogene, NF-kB subunit
NCBI Official Symbol
RELA
NCBI Official Synonym Symbols
p65; NFKB3
NCBI Protein Information
transcription factor p65
UniProt Protein Name
Transcription factor p65
UniProt Gene Name
RELA
UniProt Synonym Gene Names
NFKB3
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Product Notes
The RELA rela (Catalog #AAA111901) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The NF-kappaB p65 (Phospho-Ser276) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NF-kappaB p65 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the RELA rela for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NF-kappaB p65, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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