IF (Immunofluorescence)
(P-peptide-+ Immunofluorescence analysis of A549 cells, using PKA alpha/beta CAT (Phospho-Thr197) antibody.)
PKA alpha/beta Antibody | anti-PKA alpha/beta antibody
PKA alpha/beta CAT (Phospho-Thr197) Antibody
Gene Names
PRKACA; PKACA; PPNAD4
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
PKA alpha/beta; N/A; PKA alpha/beta CAT (Phospho-Thr197) Antibody; cAMP-dependent protein kinase catalytic subunit alpha; PKA C-alpha; PRKACA; PKACA; cAMP-dependent protein kinase catalytic subunit beta; PKA C-beta; PRKACB; anti-PKA alpha/beta antibody
Reactivity
Human, Mouse, Rat
Specificity
PKA alpha/beta CAT (Phospho-Thr197) antibody detects endogenous levels of PKA alpha/beta CAT only when phosphorylated at threonine 197.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
427
Applicable Applications for anti-PKA alpha/beta antibody
ELISA, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human PKA alpha/beta CAT around the phosphorylation site of threonine 197 (T-W-TP-L-C).
All Sites
Human: Thr197
Mouse: Thr197
Rat: Thr197
Mouse: Thr197
Rat: Thr197
Preparation and Storage
Store at-20 degree C for 1 year.
IF (Immunofluorescence)
(P-peptide-+ Immunofluorescence analysis of A549 cells, using PKA alpha/beta CAT (Phospho-Thr197) antibody.)
IF (Immunofluorescence)
(P-peptide-+ Immunofluorescence analysis of A549 cells, using PKA alpha/beta CAT (Phospho-Thr197) antibody.)
IHC (Immunohiostchemistry)
(Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using PKA alpha/beta CAT (Phospho-Thr197) antibody. Western blot analysis of extracts from mouse brain cells, using PKA alpha/beta CAT (Phospho-Thr197) antibody.)
IHC (Immunohiostchemistry)
(Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using PKA alpha/beta CAT (Phospho-Thr197) antibody. Western blot analysis of extracts from mouse brain cells, using PKA alpha/beta CAT (Phospho-Thr197) antibody.)
Application Data
(P-peptide-+)
Application Data
(P-peptide-+)
Product Categories/Family for anti-PKA alpha/beta antibody
References
Ghislaine Garrel, Endocrinology, Jun 1997; 138: 2259. W Li, Learn. Mem., Jan 1996; 2: 320-333. Sharmin Schauble, J. Biol. Chem., May 2007; 282: 14952-14959. Yong G. Wang, J. Gen. Physiol., Jan 1998; 111: 113.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39,822 Da
NCBI Official Full Name
cAMP-dependent protein kinase catalytic subunit alpha isoform 3
NCBI Official Synonym Full Names
protein kinase cAMP-activated catalytic subunit alpha
NCBI Official Symbol
PRKACA
NCBI Official Synonym Symbols
PKACA; PPNAD4
NCBI Protein Information
cAMP-dependent protein kinase catalytic subunit alpha
UniProt Protein Name
cAMP-dependent protein kinase catalytic subunit alpha
UniProt Gene Name
PRKACA
UniProt Synonym Gene Names
PKACA; PKA C-alpha
Similar Products
Product Notes
The PKA alpha/beta prkaca (Catalog #AAA109085) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The PKA alpha/beta CAT (Phospho-Thr197) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PKA alpha/beta can be used in a range of immunoassay formats including, but not limited to, ELISA, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PKA alpha/beta prkaca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PKA alpha/beta, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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