IHC (Immunohiostchemistry)
(Immunohistochemistry analysis of paraffin-embedded human brain tissue using SAPK/JNK (Phospho-Tyr185) antibody. Western blot analysis of extracts from HepG2 cells, treated with nocodazole (1ug/ml, 16hours), using SAPK/JNK (Phospho-Tyr185) antibody.)
SAPK/JNK Antibody | anti-SAPK/JNK antibody
SAPK/JNK (Phospho-Tyr185) Antibody
Gene Names
MAPK8; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
SAPK/JNK; N/A; SAPK/JNK (Phospho-Tyr185) Antibody; c-Jun N-terminal kinase 1; EC 2.7.11.24; JNK-46; JNK1-alpha-2; kinase JNK1; MAPK8; Mitogen-activated protein kinase 8; MK08; p54 gamma; PRKM8; SAPK gamma; Stress-activated protein kinase JNK1; anti-SAPK/JNK antibody
Reactivity
Human, Mouse, Rat
Specificity
SAPK/JNK (Phospho-Tyr185) antibody detects endogenous levels of SAPK/JNK only when phosphorylated at tyrosine 185.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
427
Applicable Applications for anti-SAPK/JNK antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human SAPK/JNK around the phosphorylation site of tyrosine 185 (T-P-YP-V-V).
All Sites
Human: Tyr185
Mouse: Tyr185
Rat: Tyr185
Mouse: Tyr185
Rat: Tyr185
Preparation and Storage
Store at-20 degree C for 1 year.
IHC (Immunohiostchemistry)
(Immunohistochemistry analysis of paraffin-embedded human brain tissue using SAPK/JNK (Phospho-Tyr185) antibody. Western blot analysis of extracts from HepG2 cells, treated with nocodazole (1ug/ml, 16hours), using SAPK/JNK (Phospho-Tyr185) antibody.)
IHC (Immunohiostchemistry)
(Immunohistochemistry analysis of paraffin-embedded human brain tissue using SAPK/JNK (Phospho-Tyr185) antibody. Western blot analysis of extracts from HepG2 cells, treated with nocodazole (1ug/ml, 16hours), using SAPK/JNK (Phospho-Tyr185) antibody.)
Application Data
(P-peptide-+)
Application Data
(P-peptide-+)
Product Categories/Family for anti-SAPK/JNK antibody
References
Derijard B., Cell 76:1025-1037(1994). Gupta S., EMBO J. 15:2760-2770(1996). Derijard B., Science 267:682-685(1995).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,333 Da
NCBI Official Full Name
mitogen-activated protein kinase 8 isoform beta2
NCBI Official Synonym Full Names
mitogen-activated protein kinase 8
NCBI Official Symbol
MAPK8
NCBI Official Synonym Symbols
JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2
NCBI Protein Information
mitogen-activated protein kinase 8
UniProt Protein Name
Mitogen-activated protein kinase 8
UniProt Gene Name
MAPK8
UniProt Synonym Gene Names
JNK1; PRKM8; SAPK1; SAPK1C; MAP kinase 8; MAPK 8; SAPK1c
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Product Notes
The SAPK/JNK mapk8 (Catalog #AAA111812) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The SAPK/JNK (Phospho-Tyr185) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SAPK/JNK can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the SAPK/JNK mapk8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SAPK/JNK, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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