Standard Curve (Sample)
Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) ELISA Kit | UCHL1 elisa kit
Multi-species Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) ELISA Kit
Gene Names
UCHL1; NDGOA; PARK5; PGP95; SPG79; PGP9.5; Uch-L1; HEL-117; PGP 9.5; HEL-S-53
Reactivity
Human, Mouse, Rat
Synonyms
Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1); Multi-species Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) ELISA Kit; Human/Mouse/Rat Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) (Sandwich ELISA) ELISA Kit; PARK5; PGP9.5; Uch-L1; Neuron cytoplasmic protein 9.5; Ubiquitin thioesterase L1; Ubiquitin carboxyl-terminal hydrolase isozyme L1; UCHL1 elisa kit
Reactivity
Human, Mouse, Rat
Specificity
This assay has high sensitivity for detection of UCHL1. 100% cross-reactivity of UCHL1 was observed among human, mouse and rat.
Sequence Length
223
Samples
Tissue homogenates, cell lysates, cell culture supernates and other biological fluids. due to the 96% homology of the sequence among different species, the kit can be used to detect human, mouse and rat samples
Assay Type
Quantitative Sandwich
Detection Range
0.78-50ng/mL
Sensitivity
< 0.27ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level UCHL1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level UCHL1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for UCHL1 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of UCHL1 in tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Due to the 96% homology of the sequence among different species, the kit can be used to detect human, mouse and rat samples.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to UCHL1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to UCHL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain UCHL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of UCHL1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to UCHL1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to UCHL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain UCHL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of UCHL1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24,824 Da
NCBI Official Full Name
ubiquitin carboxyl-terminal hydrolase isozyme L1
NCBI Official Synonym Full Names
ubiquitin C-terminal hydrolase L1
NCBI Official Symbol
UCHL1
NCBI Official Synonym Symbols
NDGOA; PARK5; PGP95; SPG79; PGP9.5; Uch-L1; HEL-117; PGP 9.5; HEL-S-53
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase isozyme L1
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase isozyme L1
UniProt Gene Name
UCHL1
UniProt Synonym Gene Names
UCH-L1; PGP9.5
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Product Notes
The Human, Mouse, Rat UCHL1 uchl1 (Catalog #AAA23063) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA23063 ELISA Kit recognizes Human, Mouse, Rat UCHL1. It is sometimes possible for the material contained within the vial of "Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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