Standard Curve (Sample)
Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) ELISA Kit | WNT5A elisa kit
Multi-species Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) ELISA Kit
Gene Names
WNT5A; hWNT5A
Reactivity
Human, Mouse, Rat
Synonyms
Wingless Type MMTV Integration Site Family, Member 5A (WNT5A); N/A; Multi-species Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) ELISA Kit; Human/Mouse/Rat Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) (Sandwich ELISA) ELISA Kit; WNT5A elisa kit
Reactivity
Human, Mouse, Rat
Specificity
This assay has high sensitivity for detection of WNT5A. 100% cross-reactivity of WNT5A was observed among human, mouse and rat.
Sequence Length
380
Assay Type
Quantitative Sandwich
Samples
Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.059ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level WNT5A were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level WNT5A were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for WNT5A elisa kit
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to WNT5A. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to WNT5A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain WNT5A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of WNT5A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of WNT5A in tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Due to the 98% homology of the sequence among different species, the kit can be used to detect human, mouse and rat samples.
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of WNT5A in tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Due to the 98% homology of the sequence among different species, the kit can be used to detect human, mouse and rat samples.
Product Categories/Family for WNT5A elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
40,887 Da
NCBI Official Full Name
Wingless-type MMTV integration site family, member 5A
NCBI Official Synonym Full Names
wingless-type MMTV integration site family, member 5A
NCBI Official Symbol
WNT5A
NCBI Official Synonym Symbols
hWNT5A
NCBI Protein Information
protein Wnt-5a
UniProt Protein Name
Protein Wnt-5a
UniProt Gene Name
WNT5A
UniProt Entry Name
WNT5A_HUMAN
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Product Notes
The Human, Mouse, Rat WNT5A wnt5a (Catalog #AAA20807) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA20807 ELISA Kit recognizes Human, Mouse, Rat WNT5A. It is sometimes possible for the material contained within the vial of "Wingless Type MMTV Integration Site Family, Member 5A (WNT5A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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