anti-Pig, Human CD58 Antibody | anti-CD58 antibody

Anti-Hu CD58 Purified

Cat. Num. AAA129238

• Gene Names: CD58; ag3; LFA3; LFA-3
• Reactivity: Pig, Human
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD58; N/A; Anti-Hu CD58 Purified; CD58 molecule; LFA3; AG3; anti-CD58 antibody

• Reactivity: Pig, Human
• Isotype: Mouse IgG1
• Clone Number: MEM-63
• Specificity: The antibody MEM-63 reacts with CD58 (LFA-3), a 40-70 kDa extracellular membrane glycoprotein distributed over many tissues, leukocytes, erythrocytes, endothelial cells, epithelial cells and fibroblasts.
• Form/Format: Purified; Purified by protein-A affinity chromatography.; Phosphate buffered saline (PBS), pH 8.8, 15 mM sodium azide
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CD58 antibody: FCM/FACS (Flow Cytometry)
• Immunogen: NALM-6 human pre-B cell line
• Preparation and Storage: Store at 2-8 degree C. Do not freeze.

FCM/FACS (Flow Cytometry)

(Separation of human monocytes (red-filled) from human CD58 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD58 (MEM-63) purified antibody (concentration in sample 1,67 ?g/ml, GAM APC).)

FCM/FACS (Flow Cytometry)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD58 (MEM-63) purified antibody (concentration in sample 1,67 ?g/ml, GAM APC).)

Related Product Information for anti-CD58 antibody

CD58 (LFA-3) is an immunoglobulin family adhession molecule expressed by both hematopoietic and non-hematopoietic cells (often on antigen presenting cells) and serving as ligand of CD2. This interaction is important for T cell-mediated immunity. CD58 is expressed in transmembrane form and in GPI-anchored form; the later is constitutively associated with protein kinases whereas the transmembrane form activates kinase activity upon triggering. CD58 is a powerful tool for detection of minimal residual disease in acute lymphocytic leukemia, and for evaluation of liver damage related with hepatitis B.

References

*Sheng L, Li J, Qi BT, Ji YQ, Meng ZJ, Xie M: Investigation on correlation between expression of CD58 molecule and severity of hepatitis B. World J Gastroenterol. 2006 Jul 14;12(26):4237-40., URL: https://www.ncbi.nlm.nih.gov/pubmed/16830383?ordin,*Ariel O, Kukulansky T, Raz N, Hollander N: Distinct membrane localization and kinase association of the two isoforms of CD58. Cell Signal. 2004 Jun;16(6):667-73., URL: https://www.ncbi.nlm.nih.gov/pubmed/15093607?ordin,*Veltroni M, De Zen L, Sanzari MC, Maglia O, Dworzak MN, Ratei R, Biondi A, Basso G, Gaipa G; I-BFM-ALL-FCM-MRD-Study Group: Expression of CD58 in normal, regenerating and leukemic bone marrow B cells: implications for the detection of minimal residual disease in acute lymphocytic leukemia. Haematologica. 2003 Nov;88(11):1245-52., URL: https://www.ncbi.nlm.nih.gov/pubmed/14607753?ordin,*Bayas MV, Schulten K, Leckband D: Forced detachment of the CD2-CD58 complex. Biophys J. 2003 Apr;84(4):2223-33., URL: https://www.ncbi.nlm.nih.gov/pubmed/12668431?ordin,

NCBI and Uniprot Product Information

• NCBI GI #: 221316575
• NCBI GeneID: 965
• NCBI Accession #: NP_001138294.1
• NCBI GenBank Nucleotide #: NM_001144822.1
• UniProt Accession #: P19256; Q5U053; Q6IB65; Q96KI9; A8K7G5
• Molecular Weight: 28,147 Da
• NCBI Official Full Name: lymphocyte function-associated antigen 3 isoform 2
• NCBI Official Synonym Full Names: CD58 molecule
• NCBI Official Symbol: CD58
• NCBI Official Synonym Symbols: ag3; LFA3; LFA-3
• NCBI Protein Information: lymphocyte function-associated antigen 3; surface glycoprotein LFA-3; CD58 antigen, (lymphocyte function-associated antigen 3)
• UniProt Protein Name: Lymphocyte function-associated antigen 3
• UniProt Gene Name: CD58
• UniProt Synonym Gene Names: LFA3; Ag3
• UniProt Entry Name: LFA3_HUMAN