FCM/FACS (Flow Cytometry)
(Separation of human neutrophil granulocytes (red-filled) from CD170 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD170 (1A5) APC antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)
CD170 Antibody | anti-CD170 antibody
Anti-Hu CD170 APC
Gene Names
SIGLEC5; CD170; OBBP2; CD33L2; OB-BP2; SIGLEC-5
Reactivity
Non-human primates, Human
Applications
Flow Cytometry, Functional Assay
Synonyms
CD170; N/A; Anti-Hu CD170 APC; sialic acid binding Ig like lectin 5; OBBP2; OB-BP2; SIGLEC-5; SIGLEC5; anti-CD170 antibody
Reactivity
Non-human primates, Human
Isotype
Mouse IgG1
Clone Number
1A5
Specificity
The mouse monoclonal antibody 1A5 recognizes an extracellular epitope of CD170 (Siglec-5, sialic acid binding Ig-like lectin 5), a transmembrane glycoprotein expressed strongly by neutrophils, macrophages activated during infections, monocytes, and dendritic cells. As in case with other anti-CD170 antibodies, this antibody crossreacts with Siglec-14, whose first two Ig domains are almost identical to those of CD170.
Form/Format
APC
Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 m
Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 m
Applicable Applications for anti-CD170 antibody
FCM/FACS (Flow Cytometry)
Immunogen
Fusion protein composed of human CD170 extracellular domain and Fc region of human IgG1
Preparation and Storage
Store at 2-8 degree C. Protect from prolonged exposure to light. Do not freeze.
FCM/FACS (Flow Cytometry)
(Separation of human neutrophil granulocytes (red-filled) from CD170 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD170 (1A5) APC antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Separation of human neutrophil granulocytes (red-filled) from CD170 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD170 (1A5) APC antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD170 (1A5) APC antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD170 (1A5) APC antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)
Related Product Information for anti-CD170 antibody
CD170, also known as Siglec 5 (sialic acid binding Ig-like lectin 5) is a type 1 transmembrane glycoprotein containing two cytoplasmic immunoreceptor tyrosine inhibitory motifs (ITIMs). CD170 forms homodimers and functions as an inhibitory receptor able to downregulate cell activation. It binds to alpha2,3- and alpha2,6-linked sialic acid ligands, e.g. on glycophorin A (CD235a). Aberrant expression of CD170 by CD34+ progenitor cells can be observed in case of acute myeloid leukemias.
References
*Avril T, Freeman SD, Attrill H, Clarke RG, Crocker PR: Siglec-5 (CD170) can mediate inhibitory signaling in the absence of immunoreceptor tyrosine-based inhibitory motif phosphorylation. J Biol Chem. 2005 May 20;280(20):19843-51., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=avril+t+freeman+sd,*Yokoi H, Myers A, Matsumoto K, Crocker PR, Saito H, Bochner BS: Alteration and acquisition of Siglecs during in vitro maturation of CD34+ progenitors into human mast cells. Allergy. 2006 Jun;61(6):769-76., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=yokoi+h+myers+a,*Angata T, Hayakawa T, Yamanaka M, Varki A, Nakamura M: Discovery of Siglec-14, a novel sialic acid receptor undergoing concerted evolution with Siglec-5 in primates. FASEB J. 2006 Oct;20(12):1964-73., URL: http://www.ncbi.nlm.nih.gov/pubmed/17012248,*Schuster P, Donhauser N, Pritschet K, Ries M, Haupt S, Kittan NA, Korn K, Schmidt B: Co-ordinated regulation of plasmacytoid dendritic cell surface receptors upon stimulation with herpes simplex virus type 1. Immunology. 2010 Feb;129(2):234-47., URL: http://www.ncbi.nlm.nih.gov/pubmed/19824924,
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
60,715 Da
NCBI Official Full Name
Sialic acid-binding Ig-like lectin 5
NCBI Official Synonym Full Names
sialic acid binding Ig like lectin 5
NCBI Official Symbol
SIGLEC5
NCBI Official Synonym Symbols
CD170; OBBP2; CD33L2; OB-BP2; SIGLEC-5
NCBI Protein Information
sialic acid-binding Ig-like lectin 5
UniProt Protein Name
Sialic acid-binding Ig-like lectin 5
UniProt Gene Name
SIGLEC5
UniProt Synonym Gene Names
CD33L2; OBBP2; Siglec-5; OB-BP2; OB-binding protein 2
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Product Notes
The CD170 siglec5 (Catalog #AAA129294) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hu CD170 APC reacts with Non-human primates, Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD170 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD170 siglec5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD170, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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