FCM/FACS (Flow Cytometry)
(Separation of human neutrophil granulocytes (red-filled) from human lymphocytes (black-dashed) in flow cytometry analysis (surface staining) stained using anti-human CD368 (9B9) purified antibody (concentration in sample 5 ?g/ml, GAM APC).)
CD368 Antibody | anti-CD368 antibody
Anti-Hu CD368 Purified
Gene Names
CLEC4D; MCL; MPCL; CLEC6; CLEC-6; CLECSF8
Reactivity
Non-human primates, Human
Applications
Immunoprecipitation, ELISA, Flow Cytometry, Functional Assay, Immunocytochemistry
Synonyms
CD368; N/A; Anti-Hu CD368 Purified; C-type lectin domain family 4 member D; CLEC6; CLEC4D; dectin 3; MCL; MPCL; anti-CD368 antibody
Reactivity
Non-human primates, Human
Isotype
Mouse IgG2b kappa
Clone Number
9B9
Specificity
The mouse monoclonal antibody 9B9 recognizes an extracellular epitope of CD368, a type II transmembrane protein of C lectin family, expressed mainly on monocytes and neutrophils.
Form/Format
Purified
Purified by protein-A affinity chromatography.
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Purified by protein-A affinity chromatography.
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CD368 antibody
IP (Immunoprecipitation), ELISA, FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry)
Immunogen
CD368 ectodomain fused with human Fc
Preparation and Storage
Store at 2-8 degree C. Do not freeze.
FCM/FACS (Flow Cytometry)
(Separation of human neutrophil granulocytes (red-filled) from human lymphocytes (black-dashed) in flow cytometry analysis (surface staining) stained using anti-human CD368 (9B9) purified antibody (concentration in sample 5 ?g/ml, GAM APC).)
FCM/FACS (Flow Cytometry)
(Separation of human neutrophil granulocytes (red-filled) from human lymphocytes (black-dashed) in flow cytometry analysis (surface staining) stained using anti-human CD368 (9B9) purified antibody (concentration in sample 5 ?g/ml, GAM APC).)
FCM/FACS (Flow Cytometry)
(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD368 (9B9) purified antibody (concentration in sample 5 ?g/ml, GAM APC))
FCM/FACS (Flow Cytometry)
(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD368 (9B9) purified antibody (concentration in sample 5 ?g/ml, GAM APC))
Related Product Information for anti-CD368 antibody
CD368 is an approximately 25-30 kDa C-type lectin, which serves as one of pattern recognition receptors of the innate immune system. It recognizes pathogen-associated molecular patterns of bacteria and fungi, such as alpha-mannans or trehalose 6,6´-dimycolate, and transfers the signal downstream using associated Fc receptor gamma chain. CD368 is expressed on neutrophils, monocytes, and on some populations of blood dendritic cells, but it decreases during differentiation of monocytes into dendritic cells or macrophages. Increased expression can be induced by IFN-gamma, TNF-alpha, IL-6, and IL-10. CD368 triggering leads to driving of antigen-presenting cells maturation and to promotion of T cell differentiation into Th1 and Th17.
References
*Damasceno D, Andrés MP, van den Bossche WB, Flores-Montero J, de Bruin S, Teodosio C, van Dongen JJ, Orfao A, Almeida J: Expression profile of novel cell surface molecules on different subsets of human peripheral blood antigen-presenting cells. Clin Transl Immunology 2016 Sep 16;5(9):e100., URL: https://pubmed.ncbi.nlm.nih.gov/27766148/,*Autenrieth SE, Grimm S, Rittig SM, Grünebach F, Gouttefangeas C, Bühring HJ: Profiling of primary peripheral blood- and monocyte-derived dendritic cells using monoclonal antibodies from the HLDA10 workshop in Wollongong, Australia. Clin Transl Immunology 2015 Nov 13;4(11):e50., URL: https://pubmed.ncbi.nlm.nih.gov/26682057/,*Clark G, Stockinger H, Balderas R, van Zelm MC, Zola H, Hart D, Engel P: Nomenclature of CD molecules from the Tenth human leucocyte differentiation antigen workshop. Clin Transl Immunology 2016 Jan 22;5(1):e57., URL: https://pubmed.ncbi.nlm.nih.gov/26900471/,
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
215
NCBI Official Full Name
C-type lectin domain family 4 member D
NCBI Official Synonym Full Names
C-type lectin domain family 4, member D
NCBI Official Symbol
CLEC4D
NCBI Official Synonym Symbols
MCL; MPCL; CLEC6; CLEC-6; CLECSF8
NCBI Protein Information
C-type lectin domain family 4 member D; C-type lectin receptor; macrophage C-type lectin; C-type lectin-like receptor 6; C-type lectin superfamily member 8; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 8
UniProt Protein Name
C-type lectin domain family 4 member D
UniProt Gene Name
CLEC4D
UniProt Synonym Gene Names
CLECSF8; MCL; CLEC-6
UniProt Entry Name
CLC4D_HUMAN
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Product Notes
The CD368 clec4d (Catalog #AAA129251) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hu CD368 Purified reacts with Non-human primates, Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD368 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry). Researchers should empirically determine the suitability of the CD368 clec4d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD368, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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