CD89 Antibody | anti-CD89 antibody

Anti-Hu CD89 APC

Cat. Num. AAA129222

• Gene Names: FCAR; CD89; FcalphaRI; CTB-61M7.2
• Reactivity: Human, Non-human primates
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD89; N/A; Anti-Hu CD89 APC; Fc fragment of IgA receptor; FcalphaRI; FcAR; CTB-61M7.2; anti-CD89 antibody

• Reactivity: Human, Non-human primates
• Isotype: Mouse IgG1 kappa
• Clone Number: A59
• Specificity: The mouse monoclonal antibody A59 recognizes an extracellular epitope of CD89, a 55-100 kDa glycoprotein serving as a receptor for IgA and expressed mainly on granulocytes, monocytes and macrophages.
• Form/Format: APC; Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.; Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 m

• Applicable Applications for anti-CD89 antibody: FCM/FACS (Flow Cytometry)
• Immunogen: Ag8.653 myeloma cells
• Preparation and Storage: Store at 2-8 degree C. Protect from prolonged exposure to light. Do not freeze.

FCM/FACS (Flow Cytometry)

(Separation of human monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD89 (A59) APC antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)

FCM/FACS (Flow Cytometry)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD89 (A59) APC antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)

Related Product Information for anti-CD89 antibody

CD89 (Fc-alpha-R) is a type I transmembrane glycoprotein serving as a receptor for IgA. Soluble CD89 is detectable in serum and retains its IgA binding capacity. For signal transduction the association with FcR gamma chain homodimers is needed. CD89 is expressed on granulocytes, monocytes, macrophages, dendritic cells and myeloid cell lines. Its expression is upregulated in presence of IgA immune complexes, stimulators (such as LPS, PMA), TNF alpha, IL1 beta or GM-CSF, and it is downregulated in presence of TGF beta and suramin. Binding of IgA-opsonized targets to CD89 leads to phagocytic and cytotoxic processes of the immunologic defense.

References

*Barratt J, Greer MR, Pawluczyk IZ, Allen AC, Bailey EM, Buck KS, Feehally J: Identification of a novel Fcalpha receptor expressed by human mesangial cells. Kidney Int. 2000 May;57(5):1936-48., URL: https://www.ncbi.nlm.nih.gov/pubmed/10792612,*Wu J, Ji C, Xie F, Langefeld CD, Qian K, Gibson AW, Edberg JC, Kimberly RP: FcalphaRI (CD89) alleles determine the proinflammatory potential of serum IgA. J Immunol. 2007 Mar 15;178(6):3973-82., URL: https://pubmed.ncbi.nlm.nih.gov/17339498/,*Rogers KA, Scinicariello F, Attanasio R: Identification and characterization of macaque CD89 (immunoglobulin A Fc receptor). Immunology. 2004 Oct;113(2):178-86., URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC17825,*van Dijk TB, Bracke M, Caldenhoven E, Raaijmakers JA, Lammers JW, Koenderman L, de Groot RP: Cloning and characterization of Fc alpha Rb, a novel Fc alpha receptor (CD89) isoform expressed in eosinophils and neutrophils. Blood. 1996 Dec 1;88(11):4229-38., URL: http://www.ncbi.nlm.nih.gov/pubmed/8943858,*van Egmond M, van Spriel AB, Vermeulen H, Huls G, van Garderen E, van de Winkel JG: Enhancement of polymorphonuclear cell-mediated tumor cell killing on simultaneous engagement of fcgammaRI (CD64) and fcalphaRI (CD89). Cancer Res. 2001 May 15;61(10):4055-60., URL: https://pubmed.ncbi.nlm.nih.gov/11358825/,*Honorio-França AC, Launay P, Carneiro-Sampaio MM, Monteiro RC: Colostral neutrophils express Fc alpha receptors (CD89) lacking gamma chain association and mediate noninflammatory properties of secretory IgA. J Leukoc Biol. 2001 Feb;69(2):289-96., URL: https://www.ncbi.nlm.nih.gov/pubmed/11272280,*Schenk M, Bouchon A, Seibold F, Mueller C: TREM-1--expressing intestinal macrophages crucially amplify chronic inflammation in experimental colitis and inflammatory bowel diseases. J Clin Invest. 2007 Oct;117(10):3097-106., URL: https://www.ncbi.nlm.nih.gov/pubmed/17853946,

NCBI and Uniprot Product Information

• NCBI GI #: 119860
• NCBI GeneID: 2204
• UniProt Accession #: P24071; Q13603; Q13604; Q15727; Q15728; Q1AJL7; Q1AJL8; Q1AJL9; Q53X38; Q53X39; Q92587; Q92588
• Molecular Weight: 28,343 Da
• NCBI Official Full Name: Immunoglobulin alpha Fc receptor
• NCBI Official Synonym Full Names: Fc fragment of IgA receptor
• NCBI Official Symbol: FCAR
• NCBI Official Synonym Symbols: CD89; FcalphaRI; CTB-61M7.2
• NCBI Protein Information: immunoglobulin alpha Fc receptor
• UniProt Protein Name: Immunoglobulin alpha Fc receptor
• UniProt Gene Name: FCAR
• UniProt Synonym Gene Names: CD89; IgA Fc receptor