WB (Western Blot)
( Western Blot using anti-DC-SIGNR (CLEC4M) antibody 16E7 Jurkat cell extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 16E7 at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for DC-SIGNR is 45.3kDa, though 9 other isoforms of this protein are known ranging in size from 22.4-44.7kDa (Uniprot ID: Q9H2X3). DC-SIGNR is also glycosylated at several positions. as well as multiple other isoforms.)
DC-SIGNR recombinant antibody
Anti-DC-SIGNR [16E7]
Gene Names
CLEC4M; CD299; LSIGN; CD209L; L-SIGN; DCSIGNR; HP10347; DC-SIGN2; DC-SIGNR
Reactivity
Human
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Protein A Affinity Purified
Synonyms
DC-SIGNR; N/A; Anti-DC-SIGNR [16E7]; CD299; C-type lectin domain family 4 member M; CD209 antigen-like protein 1; DC-SIGN-related protein; CLEC4M; Dendritic cell-specific ICAM-3-grabbing non-integrin 2; DC-SIGN2; Liver/lymph node-specific ICAM-3-grabbing non-integrin; L-SIGN; DC-SIGNR recombinant antibody
Reactivity
Human
Isotype
Rabbit IgG, kappa
Clone Number
16E7
Specificity
Human CD299, which is an oligomeric type II transmembrane protein with a C-type lectin extracellular domain, the expression of which is restricted to immature DC, macrophages in the lung, and endothelial cells in the liver. It binds ICAM-3 and ICAM-7 to mediate the interaction of DC with T lymphocytes and endothelial cells in the initial stages of immune response and in the migratory behavior of DC. CD299 also binds the gp120 protein of HIV and the E2 envelope protein of HCV, thereby playing a role in viral infection.
Purity/Purification
Protein A Affinity Purified
Sequence Length
399
Applicable Applications for DC-SIGNR recombinant antibody
IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry)
Buffer Composition
PBS only with 0.02% Proclin 300.
Immunogen
CD299
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
WB (Western Blot)
( Western Blot using anti-DC-SIGNR (CLEC4M) antibody 16E7 Jurkat cell extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 16E7 at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for DC-SIGNR is 45.3kDa, though 9 other isoforms of this protein are known ranging in size from 22.4-44.7kDa (Uniprot ID: Q9H2X3). DC-SIGNR is also glycosylated at several positions. as well as multiple other isoforms.)
WB (Western Blot)
( Western Blot using anti-DC-SIGNR (CLEC4M) antibody 16E7 Jurkat cell extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 16E7 at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for DC-SIGNR is 45.3kDa, though 9 other isoforms of this protein are known ranging in size from 22.4-44.7kDa (Uniprot ID: Q9H2X3). DC-SIGNR is also glycosylated at several positions. as well as multiple other isoforms.)
IF (Immunofluorescence)
( Immunofluoresence staining of fixed HeLa cells with anti-DC-SIGNR antibody 16E7 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of 16E7 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
( Immunofluoresence staining of fixed HeLa cells with anti-DC-SIGNR antibody 16E7 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of 16E7 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.)
Related Product Information for DC-SIGNR recombinant antibody
Recombinant monoclonal antibody to DC-SIGNR. Manufactured using a Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 16E7. This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Product Categories/Family for DC-SIGNR recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
24,408 Da
NCBI Official Full Name
C-type lectin domain family 4 member M
NCBI Official Synonym Full Names
C-type lectin domain family 4 member M
NCBI Official Symbol
CLEC4M
NCBI Official Synonym Symbols
CD299; LSIGN; CD209L; L-SIGN; DCSIGNR; HP10347; DC-SIGN2; DC-SIGNR
NCBI Protein Information
C-type lectin domain family 4 member M
UniProt Protein Name
C-type lectin domain family 4 member M
UniProt Gene Name
CLEC4M
UniProt Synonym Gene Names
CD209L; CD209L1; CD299; DC-SIGNR; DC-SIGN2; L-SIGN
UniProt Entry Name
CLC4M_HUMAN
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Product Notes
The DC-SIGNR clec4m (Catalog #AAA71932) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-DC-SIGNR [16E7] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DC-SIGNR can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the DC-SIGNR clec4m for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DC-SIGNR, Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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