Human Signal Transducer And Activator Of Transcription 3 (STAT3) RTU ELISA Kit | STAT3 rtu elisa kit

Human Signal Transducer And Activator Of Transcription 3 (STAT3) ELISA Kit

Cat. Num. AAA23983

• Gene Names: STAT3; APRF; HIES; ADMIO; ADMIO1
• Reactivity: Human
• Synonyms: Signal Transducer And Activator Of Transcription 3 (STAT3); N/A; Human Signal Transducer And Activator Of Transcription 3 (STAT3) ELISA Kit; APRF; Acute-Phase Response Factor; STAT3 rtu elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of STAT3. No significant cross-reactivity or interference between STAT3 and analogues was observed.
• Sequence Length: 769
• Samples: Human tissue homogenates, cell lysates, cell culture supernates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.117ng/mL

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level STAT3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level STAT3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Standard Curve (Sample)

Related Product Information for STAT3 rtu elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of STAT3 in human tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to STAT3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to STAT3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain STAT3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of STAT3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 21618338
• NCBI GeneID: 6774
• NCBI Accession #: NP_003141.2
• NCBI GenBank Nucleotide #: NM_003150.3
• UniProt Accession #: P40763; O14916; Q9BW54; A8K7B8; K7ENL3
• Molecular Weight: 83,126 Da
• NCBI Official Full Name: signal transducer and activator of transcription 3 isoform 2
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 3
• NCBI Official Symbol: STAT3
• NCBI Official Synonym Symbols: APRF; HIES; ADMIO; ADMIO1
• NCBI Protein Information: signal transducer and activator of transcription 3
• UniProt Protein Name: Signal transducer and activator of transcription 3
• UniProt Gene Name: STAT3