Standard Curve (Sample)
Human Surfactant Associated Protein A (SPA) RTU ELISA Kit | SPA rtu elisa kit
Human Surfactant Associated Protein A (SPA) ELISA Kit
Gene Names
SFTPA1; SPA; PSAP; PSPA; SP-A; SPA1; PSP-A; SFTP1; SP-A1; COLEC4; SFTPA1B
Reactivity
Human
Synonyms
Surfactant Associated Protein A (SPA); N/A; Human Surfactant Associated Protein A (SPA) ELISA Kit; PRL; SFTPA; PSAP; PSPA; SFTP; SFTPA1; COLEC4; PSP-A; SFTP1; SFTPA1B; SP-A1; Collectin-4; Alveolar proteinosis protein; 35 kDa pulmonary surfactant-associated protein; SPA rtu elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of SPA. No significant cross-reactivity or interference between SPA and analogues was observed.
Sequence Length
263
Assay Type
Quantitative Sandwich
Samples
Human serum, plasma, tissue homogenates, cell lysates, lung lavage fluid, cell culture supernates or other biological fluids
Detection Range
1.87-120ng/mL
Sensitivity
< 0.69ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SPA were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SPA were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kits: All the reagents should be kept at 4 degree C upon receipt.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for SPA rtu elisa kit
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SPA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SPA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SPA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SPA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of SPA in human serum, plasma, tissue homogenates, cell lysates, lung lavage fluid, cell culture supernates or other biological fluids.
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of SPA in human serum, plasma, tissue homogenates, cell lysates, lung lavage fluid, cell culture supernates or other biological fluids.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
27,736 Da
NCBI Official Full Name
pulmonary surfactant-associated protein A1 isoform 2
NCBI Official Synonym Full Names
surfactant protein A1
NCBI Official Symbol
SFTPA1
NCBI Official Synonym Symbols
SPA; PSAP; PSPA; SP-A; SPA1; PSP-A; SFTP1; SP-A1; COLEC4; SFTPA1B
NCBI Protein Information
pulmonary surfactant-associated protein A1
UniProt Protein Name
Pulmonary surfactant-associated protein A1
UniProt Gene Name
SFTPA1
UniProt Synonym Gene Names
COLEC4; PSAP; SFTP1; SFTPA; SFTPA1B; PSP-A; PSPA; SP-A; SP-A1
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Product Notes
The Human SPA sftpa1 (Catalog #AAA218528) is a RTU ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA218528 RTU ELISA Kit recognizes Human SPA. It is sometimes possible for the material contained within the vial of "Surfactant Associated Protein A (SPA), RTU ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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