IFN gamma elisa kit

Human IFN gamma ELISA Kit

Cat. Num. AAA58618

• Gene Names: Ifng; Ifg; IFN-g
• Reactivity: Human. No cross reactivity with other human cytokines. Cross reactivity with simian IFN-g.
• Synonyms: IFN gamma; N/A; Human IFN gamma ELISA Kit; IFN-g; IFN gamma elisa kit

• Reactivity: Human. No cross reactivity with other human cytokines. Cross reactivity with simian IFN-g.
• Specificity: The assay recognizes both natural and recombinant human IFNgamma. To define the specificity of this ELISA several proteins were tested for cross reactivity. There was no cross reactivity observed for any protein tested: IL-1alpha, IL-1beta, IL-4, IL-6, IL-8, IL-10, IL-12, IL-13, TNFalpha.
• Sequence Length: 155
• Assay Type: Qualitative Sandwich
• Samples: Supernatants, buffered solutions or serum and plasma samples
• Sensitivity: 5pg/ml

• Intra-assay Precision: Reproducibility within the assay was evaluated in three independent experiments. Each assay was carried out with 6 replicates (3 duplicates) of samples containing different concentrations of IFNgamma: 3 in human pooled serum and 2 in supernatant. 1 standard curve was run on each plate. Data below show the mean IFNgamma concentration and the coefficient of variation for each sample.
• Inter-assay Precision: Assay to assay reproducibility within one laboratory was evaluated in three independent experiments by two technicians. Each assay was carried out with 6 replicates (3 duplicates) of samples containing different concentrations of IFNgamma: 3 in human pooled serum and 2 in supernatant. 1 standard curve was run on each plate. Data below show the mean IFNgamma concentration and the coefficient of variation for each sample.

Standard Curve (Sample)

Related Product Information for IFN gamma elisa kit

Principle of the Assay: A capture Antibody highly specific for IFNgamma has been coated to the wells of the microtiter strip plate provided during manufacture. Binding of IFNgamma samples and known standards to the capture antibodies and subsequent binding of the Biotinylated anti-IFNgamma secondary antibody to the analyte is completed during the same incubation period. Any excess unbound analyte and secondary antibody is removed. The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing. A chromogen substrate is added to the wells resulting in the progressive development of a blue coloured complex with the conjugate. The colour development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced coloured complex is directly proportional to the concentration of IFNgamma present in the samples and standards. The absorbance of the colour complex is then measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of IFNgamma in any sample tested.

Intended Uses: The Human IFNgamma ELISA kit is a solid phase sandwich ELISA for the in-vitro qualitative and quantitative determination of IFNgamma in supernatants, buffered solutions or serum and plasma samples. This assay will recognise both natural and recombinant human IFNgamma.

Product Categories/Family for IFN gamma elisa kit

Human ELISA Kit

References

Alvarez-Rodriguez, L. et al., Ann Rheum Dis.,2010; 69(01): 263-269.
Bedel, R. et al., Cancer Res.,2011;71(5): 1615-1626
Botella-Carretero J. I. et al., Eur. J. Endocrinol., 2005; 153(2): 223 - 230

NCBI and Uniprot Product Information

• NCBI GI #: 33468859
• NCBI GeneID: 15978
• NCBI Accession #: NP_032363.1
• NCBI GenBank Nucleotide #: NM_008337.3
• UniProt Accession #: P01580; Q542B8
• Molecular Weight: 17,907 Da
• NCBI Official Full Name: interferon gamma
• NCBI Official Synonym Full Names: interferon gamma
• NCBI Official Symbol: Ifng
• NCBI Official Synonym Symbols: Ifg; IFN-g
• NCBI Protein Information: interferon gamma
• UniProt Protein Name: Interferon gamma
• UniProt Gene Name: Ifng
• UniProt Synonym Gene Names: IFN-gamma
• UniProt Entry Name: IFNG_MOUSE