Mouse Interleukin 1beta converting enzyme ELISA Kit | ICE elisa kit

Mouse Interleukin 1beta converting enzyme ELISA Kit

Cat. Num. AAA27292

• Reactivity: Mouse
• Synonyms: Interleukin 1beta converting enzyme; N/A; Mouse Interleukin 1beta converting enzyme ELISA Kit; Mouse Interleukin 1b converting enzyme ELISA Kit; ICE elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of ICE. No significant cross-reactivity or interference between ICE and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ICE and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for ICE elisa kit

Principle of the Assay: ICE ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ICE antibody and an ICE-HRP conjugate. The assay sample and buffer are incubated together with ICE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ICE concentration since ICE from samples and ICE-HRP conjugate compete for the anti-ICE antibody binding site. Since the number of sites is limited, as more sites are occupied by ICE from the sample, fewer sites are left to bind ICE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ICE concentration in each sample is interpolated from this standard curve.

Intended Uses: This ICE ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ICE. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for ICE elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 126352357
• NCBI GeneID: 100033888
• NCBI Accession #: NP_001075311.1
• NCBI GenBank Nucleotide #: NM_001081842.1
• Molecular Weight: 45,331 Da
• NCBI Official Full Name: caspase-1
• NCBI Official Symbol: CASP1
• NCBI Protein Information: caspase-1; ICE; p45; CASP-1; IL-1BC; IL-1 beta-converting enzyme; interleukin-1 beta convertase; interleukin-1 beta-converting enzyme; caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase)
• UniProt Protein Name: Caspase-1
• UniProt Gene Name: CASP1
• UniProt Synonym Gene Names: IL1BC; CASP-1; IL-1BC; ICE; IL-1 beta-converting enzyme
• UniProt Entry Name: CASP1_HORSE