Canine Indoleamine 2, 3-Dioxygenase ELISA Kit | IDO elisa kit
Canine Indoleamine 2, 3-Dioxygenase ELISA Kit
Reactivity
Canine
Synonyms
Indoleamine 2, 3-Dioxygenase; N/A; Canine Indoleamine 2, 3-Dioxygenase ELISA Kit; IDO elisa kit
Reactivity
Canine
Specificity
This assay has high sensitivity and excellent specificity for detection of IDO. No significant cross-reactivity or interference between IDO and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IDO and all the analogues, therefore, cross reaction may still exist in some cases.
Assay Type
Quantitative Competitive
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Sensitivity
1.0 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for IDO elisa kit
Principle of the Assay: IDO ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-IDO antibody and an IDO-HRP conjugate. The assay sample and buffer are incubated together with IDO-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IDO concentration since IDO from samples and IDO-HRP conjugate compete for the anti-IDO antibody binding site. Since the number of sites is limited, as more sites are occupied by IDO from the sample, fewer sites are left to bind IDO-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IDO concentration in each sample is interpolated from this standard curve.
Intended Uses: This IDO ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine IDO. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Intended Uses: This IDO ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine IDO. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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