Reactivity
Dog
Specificity
This assay has high sensitivity and excellent specificity for detection of canine interferon alpha -1/2. No significant cross-reactivity or interference between canine interferon alpha -1/2 and analogues was observed.
Sequence Length
167
Samples
Serum, plasma, cell culture supernates, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
12.5 pg/ml -800 pg/ml
Sensitivity
Typically less than 3.12 pg/ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Related Product Information for IFN-alpha1/2 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for interferon alpha-1/2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any interferon alpha-1/2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for interferon alpha-1/2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of interferon alpha-1/2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
IFN-alpha1