Interferon-Gamma ELISA Kit | IFN-gamma elisa kit

Interferon-Gamma (IFN-gamma), human

Cat. Num. AAA77844

• Gene Names: IFNG; IFG; IFI
• Synonyms: Interferon-Gamma; N/A; Interferon-Gamma (IFN-gamma), human; Interferon-Gamma (IFN-gamma); Interferon-Gamma (IFN-g); IFN-gamma elisa kit

• Specificity: This sandwich ELISA recognizes both natural and recombinant human IFN-gamma. This kit exhibits no detectable cross-reactivity with human; SAA, EGF, IL-6, IL-8, IL-16, M-CSF, GM-CSF, TGF-beta1, RANTES, FGF, MIP-1alpha, CRP, EPO.
• Sequence Length: 166
• Samples: Serum, plasma, Cell Culture Supernatant, and other biological fluids
• Assay Type: Sandwich
• Sensitivity: The minimum detectable dose of IFN-gamma was determined by adding two standard deviations to the mean optical density value of the 20 zero standard replicates and calculating the corresponding concentration from the standard curve. The minimum detectable dose using a standard curve generated with Calibrator Diluent I is 13.1 pg/mL and using Calibrator Diluent II is 7.5 pg/mL.

• Intra-assay Precision: To determine within-run precision, three different samples of known concentration were assayed by replicates of 20 in 1 assay.
• Inter-assay Precision: To determine between-run precision, three different samples of known concentration were assayed by replicates on 20 different assays.
• Intended Uses: This Human IFN-gamma ELISA kit is to be used for the in vitro quantitative determination of concentrations in serum, plasma, cell culture supernatant, and other biological fluids. This kit is intended FOR LABORATORY RESEARCH USE ONLY and is not for use in therapeutic procedures.

Standard Curve (Sample)

Related Product Information for IFN-gamma elisa kit

Principle of the assay: This IFN-gamma enzyme-linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal specific for IFN-gamma. Standards or samples are then added to the appropriate microtiter plate wells and incubated. IFN-gamma, if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove unbound IFN-gamma and other components of sample. In order to quantitatively determine the amount of IFN-gamma present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated monoclonal antibody specific for IFN-gamma is added to each well to "sandwich" the IFN-gamma immobilized during the first incubation. The microtiter plate then undergoes a second incubation. The wells are thoroughly washed to remove all unbound HRP-conjugated antibodies and a TMB (3,3'5, 5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IFN-gamma and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450nm +/- 2nm.

Product Categories/Family for IFN-gamma elisa kit

ELISA Kits for Human Cytokines and Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 184639
• NCBI GeneID: 3458
• Molecular Weight: 19,348 Da
• NCBI Official Full Name: interferon-gamma
• NCBI Official Synonym Full Names: interferon, gamma
• NCBI Official Symbol: IFNG
• NCBI Official Synonym Symbols: IFG; IFI
• NCBI Protein Information: interferon gamma; IFN-gamma; immune interferon
• UniProt Protein Name: Interferon gamma
• UniProt Gene Name: IFNG
• UniProt Entry Name: IFNG_HUMAN