Monkey IFN-gamma (Interferon Gamma) ELISA Kit | IFN-gamma  elisa kit

Monkey IFN-gamma (Interferon Gamma) ELISA Kit

Cat. Num. AAA250775

• Gene Names: IFNG; IFN-gamma
• Reactivity: Monkey
• Synonyms: IFN-gamma (Interferon Gamma); N/A; Monkey IFN-gamma (Interferon Gamma) ELISA Kit; IFN-gamma; IFNG; IFG; IFI; Type II Interferon; IFN-gamma elisa kit

• Reactivity: Monkey
• Specificity: This assay has high sensitivity and excellent specificity for detection of IFN-gamma. No significant cross-reactivity or interference between IFN-gamma and analogues were observed.
• Assay Type: Sandwich
• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Detection Range: 6.25-400pg/ml
• Sensitivity: 3.75pg/ml

• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Related Product Information for IFN-gamma  elisa kit

Background: Interferon-gamma (IFN-gamma), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine. Monkey IFNgamma exists as a noncovalently linked homodimer of 20-25 kDa variably glycosylated subunits. It shares 90% amino acid sequence identity with human IFN-gamma, 57-66% with bovine, canine, equine, feline, and porcine IFN-gamma, and 37-44% with cotton rat, mouse, and rat IFN- gamma. IFN- gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction. IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN -gamma functions as an antiinflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis.

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was precoated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin (HRP-SA) and Biotin Systerm (BS) was added and unbound conjugates were washed away with wash buffer. Then by twice signal amplification, TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 567316186
• NCBI GeneID: 102128291
• NCBI Accession #: NP_001274586.1
• NCBI GenBank Nucleotide #: NM_001287657.1
• UniProt Accession #: P63309; P42163; P42164
• Molecular Weight: 19,332 Da
• NCBI Official Full Name: interferon gamma
• NCBI Official Symbol: IFNG
• NCBI Official Synonym Symbols: IFN-gamma
• NCBI Protein Information: interferon gamma
• UniProt Protein Name: Interferon gamma
• UniProt Gene Name: IFNG
• UniProt Synonym Gene Names: IFN-gamma