Application Data
(Recombinant Human IFN-alpha 4 Protein cytotoxicity assay using TF-1 cells. The ED<sub>50</sub> for this effect is 0.26?1.02 ng/mL, corresponding to a specific activity of 9.80×10<sup>5</sup>~3.85×10<sup>6</sup> units/mg.)
IFN-alpha 4 Recombinant Protein | IFNA4 recombinant protein
Recombinant Human IFN-alpha 4 Protein
Purity
>90% by SDS-PAGE.
Synonyms
IFN-alpha 4; N/A; Recombinant Human IFN-alpha 4 Protein; Interferon alpha-4, IFN-alpha-4, Interferon alpha-4B, Interferon alpha-76, Interferon alpha-M1, IFNA4; IFNA4 recombinant protein
Host
HEK293 cells
Purity/Purification
>90% by SDS-PAGE.
Form/Format
Lyophilized from 0.22 um filtered solution in PBS (pH7.4). Normally 8% trehalose is added as protectant before lyophilization.
Sequence
CDLPQTHSLGNRRALILLAQMGRISHFSCLKDRHDFGFPEEEFDGHQFQKAQAISVLHEMIQQTFNLFSTEDSSAAWEQSLLEKFSTELYQQLNDLEACVIQEVGVEETPLMNEDSILAVRKYFQRITLYLTEKKYSPCAWEVVRAEIMRSLSFSTNLQKRLRRKD
Species
Human
Tag
C-His
Endotoxin
<0.1EU/ug of the protein by LAL method
Bio-Activity
Measured in a cell cytotoxicity assay using TF-1 cells. The ED50 for this effect is 0.26?1.02ng/mL, corresponding to a specific activity of 9.80×105~3.85×106 units/mg.
Preparation and Storage
Store at -20 degree C. Store the lyophilized protein at -20 degree C to -80 degree C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20 degree C for 3 months, at 2-8 degree C for up to 1 week.
After reconstitution, the protein solution is stable at -20 degree C for 3 months, at 2-8 degree C for up to 1 week.
Application Data
(Recombinant Human IFN-alpha 4 Protein cytotoxicity assay using TF-1 cells. The ED<sub>50</sub> for this effect is 0.26?1.02 ng/mL, corresponding to a specific activity of 9.80×10<sup>5</sup>~3.85×10<sup>6</sup> units/mg.)
Application Data
(Recombinant Human IFN-alpha 4 Protein cytotoxicity assay using TF-1 cells. The ED<sub>50</sub> for this effect is 0.26?1.02 ng/mL, corresponding to a specific activity of 9.80×10<sup>5</sup>~3.85×10<sup>6</sup> units/mg.)
SDS-PAGE
(Recombinant Human IFN-alpha 4 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 15-20 kDa.)
SDS-PAGE
(Recombinant Human IFN-alpha 4 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 15-20 kDa.)
Related Product Information for IFNA4 recombinant protein
IFN-alpha 4, Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.The interferons (IFN) are a family of cytokines with potent antiviral, antiproliferative and immunomodulatory properties, and are classified based on their binding specificity to cell surface receptors. The type I IFNs bind to the interferon alpha receptor (IFNAR), which consists of two subunits: IFNAR1 (alpha -subunit) and IFNAR2 (beta -subunit). This binding contributes to TNF-alpha induced signaling. Both the human and mouse genome code for more than a dozen closely related IFN-alpha subtypes and the various IFN-alphas share about 80% sequence homology among them. The mouse IFN-alpha 4 consists of 186 amino acids (aa) including a 24 aa signal peptide and a 162 aa IFN-alpha 4 mature domain. The mature mouse IFN-alpha 4 shares 58% and 76% sequence identity with mature human and rat IFN-alpha 4, respectively. The mouse IFN-alpha 4 reduces the local replication of murine cytomegalovirus in the tibia muscle. mIFN-A4 is a strong activator of Mx gene and was shown to be very effective in reducing splenomegaly.
Product Categories/Family for IFNA4 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Protein Name
Interferon alpha-4
UniProt Gene Name
IFNA4
UniProt Synonym Gene Names
IFN-alpha-4
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Product Notes
The IFNA4 ifna4 (Catalog #AAA283368) is a Recombinant Protein produced from HEK293 cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: CDLPQTHSLG NRRALILLAQ MGRISHFSCL KDRHDFGFPE EEFDGHQFQK AQAISVLHEM IQQTFNLFST EDSSAAWEQS LLEKFSTELY QQLNDLEACV IQEVGVEETP LMNEDSILAV RKYFQRITLY LTEKKYSPCA WEVVRAEIMR SLSFSTNLQK RLRRKD. It is sometimes possible for the material contained within the vial of "IFN-alpha 4, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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