Human Interferon beta ELISA Kit | IFNB1 elisa kit
Human Interferon beta ELISA Kit
Gene Names
IFNB1; IFB; IFF; IFNB; IFN-beta
Reactivity
Human
Synonyms
Interferon beta; N/A; Human Interferon beta ELISA Kit; IFN-beta; Fibroblast interferon; IFNB1; IFB; IFNB; IFNB1 elisa kit
Reactivity
Human
Specificity
Recombinant and natural Human Interferon beta, IFN-beta
Sequence Length
187
Samples
Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
Detection Range
15.6-1000 pg/mL
Sensitivity
<7.8 pg/mL
Intra-assay Precision
<=5.9%
Inter-assay Precision
<=9.8%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Standard Curve (Sample)
Related Product Information for IFNB1 elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,294 Da
NCBI Official Full Name
interferon beta
NCBI Official Synonym Full Names
interferon, beta 1, fibroblast
NCBI Official Symbol
IFNB1
NCBI Official Synonym Symbols
IFB; IFF; IFNB; IFN-beta
NCBI Protein Information
interferon beta
UniProt Protein Name
Interferon beta
UniProt Gene Name
IFNB1
UniProt Synonym Gene Names
IFB; IFNB; IFN-beta
UniProt Entry Name
IFNB_HUMAN
