Standard Curve (Sample)
Rat IFN gamma ELISA Kit | IFNG elisa kit
Rat IFN gamma PicoKine ELISA Kit
Gene Names
Ifng; IFNG2
Reactivity
Rat
Synonyms
IFN gamma; N/A; Rat IFN gamma PicoKine ELISA Kit; Interferon gamma; interferon, gamma; IFNG; IFG; IFI; IFN Gamma; IFN Immune; IFN-gamma; IFNG_HUMAN; Immune Interferon; Interferon Gamma Precursor; Macrophage Activating Factor; MAF; T Cell Interferon; Type II Interferon; IFNG elisa kit
Reactivity
Rat
Specificity
Natural and recombinant Rat Ifng
Sequence Length
156
Samples
Cell culture supernatants and serum
Detection Range
31.2 pg/ml - 2,000 pg/ml
Sensitivity
<6 pg/ml
Intra-assay Precision
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for IFNG elisa kit
Background: Interferon-gamma (IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer (NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.
Principle of the Assay: The Picokine™ Rat Ifng Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Rat Ifng with a 96-well strip plate that is pre-coated with antibody specific for Ifng. The detection antibody is a biotinylated antibody specific for Ifng. The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Rat Ifng with immunogen: Expression system for standard: E.coli; Immunogen sequence: E23-C156. The kit is analytically validated with ready-to-use reagents. To measure Rat Ifng, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Rat Ifng in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Rat Ifng in the sample.
Principle of the Assay: The Picokine™ Rat Ifng Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Rat Ifng with a 96-well strip plate that is pre-coated with antibody specific for Ifng. The detection antibody is a biotinylated antibody specific for Ifng. The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Rat Ifng with immunogen: Expression system for standard: E.coli; Immunogen sequence: E23-C156. The kit is analytically validated with ready-to-use reagents. To measure Rat Ifng, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Rat Ifng in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Rat Ifng in the sample.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
17,918 Da
NCBI Official Full Name
Interferon gamma
NCBI Official Synonym Full Names
interferon gamma
NCBI Official Symbol
Ifng
NCBI Official Synonym Symbols
IFNG2
NCBI Protein Information
interferon gamma; IFN-gamma
UniProt Protein Name
Interferon gamma
UniProt Gene Name
Ifng
UniProt Synonym Gene Names
IFN-gamma
UniProt Entry Name
IFNG_RAT
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Product Notes
The Rat IFNG ifng (Catalog #AAA11587) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA11587 ELISA Kit recognizes Rat IFNG. It is sometimes possible for the material contained within the vial of "IFN gamma, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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