Standard Curve (Sample)
Human IL-29 ELISA Kit | IFNL1 elisa kit
Human IL-29 PicoKine ELISA Kit
Gene Names
IFNL1; IL29; IL-29
Reactivity
Human
Synonyms
IL-29; N/A; Human IL-29 PicoKine ELISA Kit; Interferon lambda-1; interferon, lambda 1; Cytokine ZCYTO21; IFN lambda 1; IFN-lambda-1; IFNL1; IL 29; IL29; IL29_HUMAN; Interferon lambda 1; Interferon, lambda 1; Interleukin 29; Interleukin-29; IFNL1 elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human IFNL1
Sequence Length
200
Samples
Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Detection Range
62.5 pg/ml - 4,000 pg/ml
Sensitivity
<10 pg/ml
Intra-assay Precision
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for IFNL1 elisa kit
Background: Interferon lambda-1, also called Interleukin-29 (IL-29) is a protein that in humans is encoded by the IL29 gene. IL-29 is a member of the helical cytokine family and is a type III interferon. This gene is mapped to 19q13.2. It is a cytokine with immunomodulatory activity. This gene may play a role in antiviral immunity. It can up-regulates MHC class I antigen expression. It is the ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1. The ligand/receptor complex seems to signal through the Jak-STAT pathway. IL-29 plays an important role in host defenses against microbes and its gene is highly upregulated in cells infected with viruses.
Principle of the Assay: The Picokine™ Human IFNL1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human IFNL1 with a 96-well strip plate that is pre-coated with antibody specific for IFNL1. The detection antibody is a biotinylated antibody specific for IFNL1. The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human IFNL1 with immunogen: Expression system for standard: NS0; Immunogen sequence: G20-T200. The kit is analytically validated with ready-to-use reagents. To measure Human IFNL1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human IFNL1 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human IFNL1 in the sample.
Principle of the Assay: The Picokine™ Human IFNL1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human IFNL1 with a 96-well strip plate that is pre-coated with antibody specific for IFNL1. The detection antibody is a biotinylated antibody specific for IFNL1. The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human IFNL1 with immunogen: Expression system for standard: NS0; Immunogen sequence: G20-T200. The kit is analytically validated with ready-to-use reagents. To measure Human IFNL1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human IFNL1 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human IFNL1 in the sample.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
21,898 Da
NCBI Official Full Name
Interferon lambda-1
NCBI Official Synonym Full Names
interferon, lambda 1
NCBI Official Symbol
IFNL1
NCBI Official Synonym Symbols
IL29; IL-29
NCBI Protein Information
interferon lambda-1; IFN-lambda-1; interleukin-29; cytokine Zcyto21; interferon-lambda-1; interleukin 29 (interferon, lambda 1)
UniProt Protein Name
Interferon lambda-1
UniProt Gene Name
IFNL1
UniProt Synonym Gene Names
IL29; ZCYTO21; IFN-lambda-1; IL-29
UniProt Entry Name
IFNL1_HUMAN
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Product Notes
The Human IFNL1 ifnl1 (Catalog #AAA11613) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA11613 ELISA Kit recognizes Human IFNL1. It is sometimes possible for the material contained within the vial of "IL-29, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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