Rat IGF-1 ELISA Kit | IGF1 elisa kit

Rat IGF-1 PicoKine ELISA Kit

Cat. Num. AAA11579

• Reactivity: Rat
• Synonyms: IGF-1; N/A; Rat IGF-1 PicoKine ELISA Kit; Insulin-like growth factor I; insulin-like growth factor 1 (somatomedin C); IGF-I; Igf1; IGF1_HUMAN; Mechano growth factor; MGF; Somatomedin-C; IGF1 elisa kit

• Reactivity: Rat
• Specificity: Natural and recombinant Rat Igf1
• Sequence Length: 153
• Samples: Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
• Detection Range: 62.5 pg/ml - 4,000 pg/ml
• Sensitivity: <5 pg/ml

• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Standard Curve (Sample)

Related Product Information for IGF1 elisa kit

Background: Insulin-like growth factor 1 (IGF-1) that was once called somatomedin C, is a polypeptide protein hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. Human IGF1 is a single chain 70-amino acid polypeptide cross-linked by 3 disulfide bridges, with a calculated molecular mass of 7.6 kD. The IGF1 gene, mapped on 12q22-q24.1, contains 5 exons. Exons 1-4 encode the 195-amino acid precursor (IGF1B), and exons 1, 2, 3, and 5 encode the 153-residue peptide (IGF1A). The structure of IGF1 resembles that of IGF2. And the IGF1 and IGF2 genes have complex structures with multiple promoters. The expression of both genes is regulated at the levels of transcription, RNA processing, and translation. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. Moreover, approximately 98% of IGF-1 is always bound to one of 6 binding proteins (IGF-BP). Furthermore, IGF-1 is one of the most potent natural activators of the AKT signaling pathway, a stimulator of cell growth and multiplication and a potent inhibitor of programmed cell death.

Principle of the Assay: The Picokine™ Rat Igf1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Rat Igf1 with a 96-well strip plate that is pre-coated with antibody specific for Igf1. The detection antibody is a biotinylated antibody specific for Igf1. The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat specific for IGF-1. The kit includes Rat Igf1 protein as standards. To measure Rat Igf1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Rat Igf1 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Rat Igf1 in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 124262
• NCBI GeneID: 24482
• UniProt Accession #: P08025; P08024
• Molecular Weight: 17,831 Da
• NCBI Official Full Name: Insulin-like growth factor I
• NCBI Official Synonym Full Names: insulin-like growth factor 1
• NCBI Official Symbol: Igf1
• NCBI Protein Information: insulin-like growth factor I; IGF-I; somatomedin
• UniProt Protein Name: Insulin-like growth factor I
• UniProt Gene Name: Igf1
• UniProt Synonym Gene Names: Igf-1; IGF-I
• UniProt Entry Name: IGF1_RAT