Insulin Like Growth Factor-I Recombinant Protein | IGF-I recombinant protein

Human Insulin Like Growth Factor-I Recombinant

Cat. Num. AAA76064

• Purity: >95%, as determined by SDS-PAGE and HPLC
• Synonyms: Insulin Like Growth Factor-I; N/A; Human Insulin Like Growth Factor-I Recombinant; igf-1; insulin like; igf1; igf-i; IGF-I recombinant protein

• Host: E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
• Purity/Purification: >95%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant Human IGF1 was lyophilized from a 0.2 um filtered PBS solution, pH 7.4.
• Sequence: MGKISSLPTQ LFKCCFCDFL KVKMHTMSSS HLFYLALCLL TFTSSATAGP ETLCGAELVD ALQFVCGDRG FYFNKPTGYG SSSRRAPQTG IVDECCFRSC DLRRLEMYCA PLKPAKSARS VRAQRHTDMP KTQKEVHLKN ASRGSAGNKN YRM
• Sequence Length: 153

• Domain: Insulin
• Host Note: Optimized DNA sequence encoding Human Insulin Growth Factor -1mature chain was expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Molecular Weight Note: Native Human Insulin Growth Factor-1 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 8 kDa.; Recombinant Human IGF-1 is a disulfide-linked monomeric protein consisting of 71 amino acid residue subunits. IGF-1 migrates as an approximately 8 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for IGF-I recombinant protein

Insulin-like growth factor I, also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Human IGF-I is synthesized as two precursor isoforms with N- and alternate C- terminal propeptides (1). These isoforms are differentially expressed by various tissues (1). The 7.6 kDa mature IGF-I is identical between isoforms and is generated by proteolytic removal of the N- and C- terminal regions. Mature human IGF-I shares 94% and 96% aa sequence identity with mouse and rat IGF-I, respectively (2), and exhibits cross-species activity. It shares 64% aa sequence identity with mature human IGF-II. Circulating IGF-I is produced by hepatocytes, while local IGF-I is produced by many other tissues in which it has paracrine effects.

Product Categories/Family for IGF-I recombinant protein

Cytokines

References

• statement by the growth hormone research society on the gh/IGF-I axis in extending health span .J Gerontol A Biol Sci Med Sci, Oct 2009; 64A: 1039 - 1044.
• partial primary deficiency of insulin-like growth factor (igf) -i activity associated with igf1 mutation demonstrates its critical role in growth and brain development .J. Clin. Endocrinol. Metab., Sep 2009; 10.1210/jc.2009-0452.
• myostatin inhibits IGF-I induced myotube hypertrophy through akt .Am J Physiol Cell Physiol, Sep 2009; 10.1152/ajpcell.00043.2009.
• the insulin-like growth factor (igf) -i e-peptides modulate cell entry of the mature IGF-I protein .Mol. Biol. Cell, Sep 2009; 20: 3810 - 3817.
• elevated levels of insulin-like growth factor (igf) -i in serum rescue the severe growth retardation of IGF-I null mice .Endocrinology, Sep 2009; 150: 4395 - 4403.