IGFBP-1 recombinant protein

Recombinant human IGFBP-1 protein

Cat. Num. AAA48429

• Gene Names: IGFBP1; AFBP; IBP1; PP12; IGF-BP25; hIGFBP-1
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: IGFBP-1; N/A; Recombinant human IGFBP-1 protein; insulin like growth factor binding protein 1; IGFBP1; AFBP; hIGFBP-1; IBP1; IGF-BP25; PP12; IGF-binding protein 1; Placental protein 12; amniotic fluid binding protein; alpha-pregnancy-associated endometrial globulin; IGFBP-1 recombinant protein

• Host: HEK293
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
• Concentration: 0.5mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 26-259aa
• Sequence: APWQCAPCSA EKLALCPPVS ASCSEVTRSA GCGCCPMCAL PLGAACGVAT ARCARGLSCR ALPGEQQPLH ALTRGQGACV QESDASAPHA AEAGSPESPE STEITEEELL DNFHLMAPSE EDHSILWDAI STYDGSKALH VTNIKKWKEP CRIELYRVVE SLAKAQETSG EEISKFYLPN CNKNGFYHSR QCETSMDGEA GLCWCVYPWN GKRIPGSPEI RGDPNCQIYF NVQN

• Applicable Applications for IGFBP-1 recombinant protein: Bioactivity, SDS-PAGE
• Species: Human
• Tag: Non-Tagged
• Predicted Nterm: Ala26
• Endotoxin: < 1 EU per 1ug of protein (determined by LAL method)
• Bioactivity: Measured by its ability to inhibit proliferation using MCF-7 human breast cancer cells in the presence of Human IGF-1. The ED50 range <=3 ug/ml.
• Preparation and Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Bioactivity

(MagnifyHuman IGFBP-1 inhibit proliferation using MCF-7 human breast cancer cells in the presence of Human IGF-1. The ED50 range )

SDS-PAGE

(Magnify3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for IGFBP-1 recombinant protein

IGFBP-1(Insulin-like growth factor-binding protein 1), also known as PP12(placental protein 12), is a member of the Insulin-like growth factor-binding protein family. This protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. IGFBP-1 is expressed in liver, decidua, kidneys and is the most abundant IGFBP in amniotic fluid. Serum levels of IGFBP-1 are lowest after meals. Hepatocyte production of IGFBP-1 is regulated at the transcriptional level due to the affects of insulin and corticosteriods. Recombinant human IGFBP-1 was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Categories/Family for IGFBP-1 recombinant protein

Signal Transduction

References

Jones, J.I. and D.R. Clemmons (1995) Endocrine Rev. 16:3-34.;; Kelley, K.M., et al, (1996) Int. J. Biochem. Cell Biol. 28:619-637.;; J.Ekstrand., et al, (1990) Genomics. 6:413-418.

NCBI and Uniprot Product Information

• NCBI GI #: 4504615
• NCBI GeneID: 3484
• NCBI Accession #: NP_000587.1
• NCBI GenBank Nucleotide #: NM_000596.2
• UniProt Accession #: P08833; Q8IYP5; A4D2F4; D3DVL9
• Molecular Weight: 27,904 Da
• NCBI Official Full Name: insulin-like growth factor-binding protein 1
• NCBI Official Synonym Full Names: insulin-like growth factor binding protein 1
• NCBI Official Symbol: IGFBP1
• NCBI Official Synonym Symbols: AFBP; IBP1; PP12; IGF-BP25; hIGFBP-1
• NCBI Protein Information: insulin-like growth factor-binding protein 1; IBP-1; IGF-binding protein 1; IGFBP-1; alpha-pregnancy-associated endometrial globulin; amniotic fluid binding protein; binding protein-25; binding protein-26; binding protein-28; growth hormone independent-bi
• UniProt Protein Name: Insulin-like growth factor-binding protein 1
• UniProt Gene Name: IGFBP1
• UniProt Synonym Gene Names: IBP1; IBP-1; IGF-binding protein 1; IGFBP-1; PP12
• UniProt Entry Name: IBP1_HUMAN