Monkey Insulin Like Growth Factor Binding Protein 2 ELISA Kit | IGFBP2 elisa kit
Monkey Insulin Like Growth Factor Binding Protein 2 ELISA Kit
Gene Names
IGFBP2; IBP2; IGF-BP53
Reactivity
Monkey
Synonyms
Insulin Like Growth Factor Binding Protein 2; N/A; Monkey Insulin Like Growth Factor Binding Protein 2 ELISA Kit; IGFBP2 elisa kit
Reactivity
Monkey
Specificity
This assay has high sensitivity and excellent specificity for detection of IGFBP2. No significant cross-reactivity or interference between IGFBP2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IGFBP2 and all the analogues, therefore, cross reaction may still exist in some cases.
Assay Type
Quantitative Competitive
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Sensitivity
0.1 ng/ml
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for IGFBP2 elisa kit
Principle of the Assay: IGFBP2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-IGFBP2 antibody and an IGFBP2-HRP conjugate. The assay sample and buffer are incubated together with IGFBP2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IGFBP2 concentration since IGFBP2 from samples and IGFBP2-HRP conjugate compete for the anti-IGFBP2 antibody binding site. Since the number of sites is limited, as more sites are occupied by IGFBP2 from the sample, fewer sites are left to bind IGFBP2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IGFBP2 concentration in each sample is interpolated from this standard curve.
Intended Uses: This IGFBP2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey IGFBP2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Intended Uses: This IGFBP2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey IGFBP2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Product Categories/Family for IGFBP2 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
34,814 Da
NCBI Official Full Name
Insulin-like growth factor-binding protein 2
NCBI Official Synonym Full Names
insulin-like growth factor binding protein 2, 36kDa
NCBI Official Symbol
IGFBP2
NCBI Official Synonym Symbols
IBP2; IGF-BP53
NCBI Protein Information
insulin-like growth factor-binding protein 2; IBP-2; IGFBP-2; IGF-binding protein 2
UniProt Protein Name
Insulin-like growth factor-binding protein 2
UniProt Gene Name
IGFBP2
UniProt Synonym Gene Names
BP2; IBP2; IBP-2; IGF-binding protein 2; IGFBP-2
UniProt Entry Name
IBP2_HUMAN
