Standard Curve (Sample)
Canine Insulin Like Growth Factor Binding Protein 4 ELISA Kit | IGFBP4 elisa kit
Canine Insulin Like Growth Factor Binding Protein 4 ELISA Kit
Gene Names
IGFBP4; BP-4; IBP4; IGFBP-4; HT29-IGFBP
Reactivity
Canine
Synonyms
Insulin Like Growth Factor Binding Protein 4; N/A; Canine Insulin Like Growth Factor Binding Protein 4 ELISA Kit; IGFBP4 elisa kit
Reactivity
Canine
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
50-1000pg/mL
Sensitivity
1.0pg/mL
Intended Uses
This IGFBP4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine IGFBP4. This ELISA kit for research use only, not for therapeutic applications
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for IGFBP4 elisa kit
Principle of the Assay: IGFBP4 ELISAkit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IGFBP4 antibody and an IGFBP4-HRP conjugate. The assay sample and buffer are incubated together with IGFBP4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IGFBP4 concentration since IGFBP4 from samples and IGFBP4-HRP conjugate compete for the anti-IGFBP4 antibody binding site. Since the number of sites is limited, as more sites are occupied by IGFBP4 from the sample, fewer sites are left to bind IGFBP4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IGFBP4 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for IGFBP4 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27,934 Da
NCBI Official Full Name
insulin-like growth factor-binding protein 4
NCBI Official Synonym Full Names
insulin-like growth factor binding protein 4
NCBI Official Symbol
IGFBP4
NCBI Official Synonym Symbols
BP-4; IBP4; IGFBP-4; HT29-IGFBP
NCBI Protein Information
insulin-like growth factor-binding protein 4; IBP-4; IGF-binding protein 4
UniProt Protein Name
Insulin-like growth factor-binding protein 4
UniProt Gene Name
IGFBP4
UniProt Synonym Gene Names
IBP4; IBP-4; IGF-binding protein 4; IGFBP-4
UniProt Entry Name
IBP4_HUMAN
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Product Notes
The Canine IGFBP4 igfbp4 (Catalog #AAA88929) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA88929 ELISA Kit recognizes Canine IGFBP4. It is sometimes possible for the material contained within the vial of "Insulin Like Growth Factor Binding Protein 4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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