Sheep Insulin Like Growth Factor Binding Protein 5 ELISA Kit | IGFBP5 elisa kit

Sheep Insulin Like Growth Factor Binding Protein 5 ELISA Kit

Cat. Num. AAA88635

• Gene Names: Igfbp5; IGF-BP5
• Reactivity: Sheep
• Synonyms: Insulin Like Growth Factor Binding Protein 5; N/A; Sheep Insulin Like Growth Factor Binding Protein 5 ELISA Kit; IGFBP5 elisa kit

• Reactivity: Sheep
• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL.

• Intended Uses: This IGFBP5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep IGFBP5. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for IGFBP5 elisa kit

Principle of the assay: IGFBP5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IGFBP5 antibody and an IGFBP5-HRP conjugate. The assay sample and buffer are incubated together with IGFBP5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IGFBP5 concentration since IGFBP5 from samples and IGFBP5-HRP conjugate compete for the anti-IGFBP5 antibody binding site. Since the number of sites is limited, as more sites are occupied by IGFBP5 from the sample, fewer sites are left to bind IGFBP5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IGFBP5 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for IGFBP5 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 6981088
• NCBI GeneID: 25285
• NCBI Accession #: NP_036949.1
• NCBI GenBank Nucleotide #: NM_012817.1
• Molecular Weight: 30,298 Da
• NCBI Official Full Name: insulin-like growth factor-binding protein 5
• NCBI Official Synonym Full Names: insulin-like growth factor binding protein 5
• NCBI Official Symbol: Igfbp5
• NCBI Official Synonym Symbols: IGF-BP5
• NCBI Protein Information: insulin-like growth factor-binding protein 5; IBP-5; IGFBP-5; IGF-binding protein 5
• UniProt Protein Name: Insulin-like growth factor-binding protein 5
• UniProt Gene Name: Igfbp5
• UniProt Synonym Gene Names: Igfbp-5; IBP-5; IGF-binding protein 5; IGFBP-5
• UniProt Entry Name: IBP5_RAT