Sheep Insulin Like Growth Factor Binding Protein 6 ELISA Kit | IGFBP6 elisa kit

Sheep Insulin Like Growth Factor Binding Protein 6 ELISA Kit

Cat. Num. AAA91877

• Gene Names: IGFBP6; IBP6
• Reactivity: Sheep
• Synonyms: Insulin Like Growth Factor Binding Protein 6; N/A; Sheep Insulin Like Growth Factor Binding Protein 6 ELISA Kit; IGFBP6 elisa kit

• Reactivity: Sheep
• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.

• Intended Uses: This IGFBP6 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep IGFBP6. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for IGFBP6 elisa kit

Principle of the assay: IGFBP6 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IGFBP6 antibody and an IGFBP6-HRP conjugate. The assay sample and buffer are incubated together with IGFBP6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IGFBP6 concentration since IGFBP6 from samples and IGFBP6-HRP conjugate compete for the anti-IGFBP6 antibody binding site. Since the number of sites is limited, as more sites are occupied by IGFBP6 from the sample, fewer sites are left to bind IGFBP6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IGFBP6 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for IGFBP6 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 11321593
• NCBI GeneID: 3489
• NCBI Accession #: NP_002169.1
• NCBI GenBank Nucleotide #: NM_002178.2
• Molecular Weight: 25,322 Da
• NCBI Official Full Name: insulin-like growth factor-binding protein 6
• NCBI Official Synonym Full Names: insulin-like growth factor binding protein 6
• NCBI Official Symbol: IGFBP6
• NCBI Official Synonym Symbols: IBP6
• NCBI Protein Information: insulin-like growth factor-binding protein 6; IBP-6; IGFBP-6; IGF binding protein 6; IGF-binding protein 6
• UniProt Protein Name: Insulin-like growth factor-binding protein 6
• UniProt Gene Name: IGFBP6
• UniProt Synonym Gene Names: IBP6; IBP-6; IGF-binding protein 6; IGFBP-6
• UniProt Entry Name: IBP6_HUMAN