Standard Curve (Sample)
Interleukin-13 ELISA Kit | IL-13 elisa kit
Interleukin-13 (IL-13)
Gene Names
IL13; P600; IL-13
Synonyms
Interleukin-13; N/A; Interleukin-13 (IL-13); IL-13 elisa kit
Specificity
This sandwich ELISA recognises both natural and recombinant human IL-13 Ra2. This kit has been tested and exhibited no significant cross-reactivity with following cytokines and growth factors: IL-1 alpha, IL-1beta, IL-2, ?IL-2sRa, IL-4,?IL-5, IL-6, IL-7, IL- 8, IL-10, IL-11, IL-12, Il-17A, IL-15, FGF basic, GM-CSF, IFN-gamma, M-CSF, MCP- 1(MCAF), MCP3, EGF, TNF- alpha, TNF- beta.
Sequence Length
146
Samples
Cell Culture Supernatant
Assay Type
Sandwich
Intra-assay Precision
To determine within-run precision, three different samples of known concentration were assayed by replicates of 8 in 1 assay.
Inter-assay Precision
To determine between-run precision, three different samples of known concentration were assayed by replicates on 8 different assays.
Intended Uses
This Human Interleukin 13 ELISA Kit is to be used for the in vitro quantitative determination of human interleukin 13 concentrations in cell culture supernatant. This kit is intended FOR LABORATORY RESEARCH USE ONLY and is not for use in therapeutic procedures.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for IL-13 elisa kit
Principle of the assay: This assay applies the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific to IL-13 has been pre-coated onto a microplate. Standards and samples are then added to the appropriate plate wells with a biotin-conjugated antibody preparation specific for IL-13 and incubated. IL-13 if present, will bind and become immobilized by the antibody pre-coated on the wells and then be "sandwiched" by biotin conjugate. Avidin is a tetramer containing four identical subunits that each has a high affinity-binding site for biotin. After washing away any unbound substances, avidin- Horseradish Peroxidase (HRP) will be added to each well and incubated. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and only those wells that contain IL-13, biotin conjugated antibody and enzymeconjugated Avidin will exhibit a change in colour. The colour development is stopped and the intensity of the colour is measured. In order to measure the concentration of IL-13 in the samples, this package of reagent includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing.) According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus IL-13 concentration. The concentration of IL-13 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Background: IL-13 is a 10kD unglycosylated protein, secreted mainly by activated T helper 2 cells. IL- 13 binds to IL-4Ralpha in addition to IL-13Ralpha1 and IL-13Ralpha2. And the function of IL-13 partially overlaps with IL-4. Both IL-4 and IL-13 link to signal transducer and activator of transcription 6 (STAT6) through the receptor activation. IL-13 can induce B-cell proliferation and IgE classs switching. However, IL-13 is less competent in this function comparing with IL-4. Unlike IL-4, IL-13 does not play an important role in hematopoietic cell shift, such as naïve T helper 0 differentiation to T helper 2. IL-I3 signaling is significant in inducing physiological changes to non-immuno cells in parasitized organs. For example, IL-13 induces enhanced gut contractions and glycoprotein hypersecretion which lead to the detachment of helminthes from gut wall. Eggs produced by Schistosoma mansoni can be limited inside granulomas formed through IL-13 induced cell changes. To organisms resided inside cells, IL-13 seemed to inhibit the clearance from host cell through antagonizing Th1 responses. IL-13 is also associated with many features of the allergic airway diseases, including mucus hypersecretion, goblet metaplasia, chemokine induction and recruitment of effector cells. All of them contribute to airway obstruction. Polymorphisms in IL-13 gene have been found to link to increased eosinophil count, serum total IgE and high risk of asthma.
Background: IL-13 is a 10kD unglycosylated protein, secreted mainly by activated T helper 2 cells. IL- 13 binds to IL-4Ralpha in addition to IL-13Ralpha1 and IL-13Ralpha2. And the function of IL-13 partially overlaps with IL-4. Both IL-4 and IL-13 link to signal transducer and activator of transcription 6 (STAT6) through the receptor activation. IL-13 can induce B-cell proliferation and IgE classs switching. However, IL-13 is less competent in this function comparing with IL-4. Unlike IL-4, IL-13 does not play an important role in hematopoietic cell shift, such as naïve T helper 0 differentiation to T helper 2. IL-I3 signaling is significant in inducing physiological changes to non-immuno cells in parasitized organs. For example, IL-13 induces enhanced gut contractions and glycoprotein hypersecretion which lead to the detachment of helminthes from gut wall. Eggs produced by Schistosoma mansoni can be limited inside granulomas formed through IL-13 induced cell changes. To organisms resided inside cells, IL-13 seemed to inhibit the clearance from host cell through antagonizing Th1 responses. IL-13 is also associated with many features of the allergic airway diseases, including mucus hypersecretion, goblet metaplasia, chemokine induction and recruitment of effector cells. All of them contribute to airway obstruction. Polymorphisms in IL-13 gene have been found to link to increased eosinophil count, serum total IgE and high risk of asthma.
Product Categories/Family for IL-13 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15,816 Da
NCBI Official Full Name
interleukin-13
NCBI Official Synonym Full Names
interleukin 13
NCBI Official Symbol
IL13
NCBI Official Synonym Symbols
P600; IL-13
NCBI Protein Information
interleukin-13
UniProt Protein Name
Interleukin-13
UniProt Gene Name
IL13
UniProt Entry Name
IL13_HUMAN
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Product Notes
The IL-13 il13 (Catalog #AAA77878) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Interleukin-13, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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