Standard Curve (Sample)
Mouse Soluble Interleukin 2a receptor ELISA Kit | IL-2sRa elisa kit
Mouse Soluble Interleukin 2a receptor ELISA Kit
Gene Names
IL2RA; p55; CD25; IL2R; TCGFR; IDDM10
Reactivity
Mouse
Synonyms
Soluble Interleukin 2a receptor; Mouse Soluble Interleukin 2a receptor ELISA Kit; IL-2sRa elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of sIL-2AR. No significant cross-reactivity or interference between sIL-2AR and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between sIL-2AR and all the analogues, therefore, cross reaction may still exist in some cases.
Assay Type
Quantitative Competitive
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Sensitivity
1.0 pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for IL-2sRa elisa kit
Principle of the Assay: sIL-2AR ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-sIL-2AR antibody and an sIL-2AR-HRP conjugate. The assay sample and buffer are incubated together with sIL-2AR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the sIL-2AR concentration since sIL-2AR from samples and sIL-2AR-HRP conjugate compete for the anti-sIL-2AR antibody binding site. Since the number of sites is limited, as more sites are occupied by sIL-2AR from the sample, fewer sites are left to bind sIL-2AR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sIL-2AR concentration in each sample is interpolated from this standard curve.
Intended Uses: This sIL-2AR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse sIL-2AR. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Intended Uses: This sIL-2AR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse sIL-2AR. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Product Categories/Family for IL-2sRa elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
30,819 Da
NCBI Official Full Name
interleukin 2 receptor, alpha, isoform CRA_a, partial
NCBI Official Synonym Full Names
interleukin 2 receptor, alpha
NCBI Official Symbol
IL2RA
NCBI Official Synonym Symbols
p55; CD25; IL2R; TCGFR; IDDM10
NCBI Protein Information
interleukin-2 receptor subunit alpha; TAC antigen; IL-2R subunit alpha; IL-2 receptor subunit alpha
UniProt Protein Name
Interleukin-2 receptor subunit alpha
UniProt Gene Name
IL2RA
UniProt Synonym Gene Names
IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA
UniProt Entry Name
IL2RA_HUMAN
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Product Notes
The Mouse IL-2sRa il2ra (Catalog #AAA16410) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA16410 ELISA Kit recognizes Mouse IL-2sRa. It is sometimes possible for the material contained within the vial of "Soluble Interleukin 2a receptor, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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