Human Interleukin 33/ST2 ELISA Kit | IL-33/ST2 elisa kit

Human Interleukin 33/ST2 ELISA Kit

Cat. Num. AAA16681

• Gene Names: IL33; DVS27; IL1F11; NF-HEV; NFEHEV; C9orf26; DKFZp586H0523; RP11-575C20.2
• Reactivity: Human
• Synonyms: Interleukin 33/ST2; Human Interleukin 33/ST2 ELISA Kit; Human Interleukin 33/ST2ELISA Kit; Interleukin 33/ST2 ELISA Kit (Human); IL-33/ST2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of L-33/ST2. No significant cross-reactivity or interference between L-33/ST2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between L-33/ST2 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Sandwich
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0pg/mL

• Preparation and Storage: Store all reagents at 2-8 degree C

Standard Curve (Sample)

Related Product Information for IL-33/ST2 elisa kit

Principle of the Assay: L-33/ST2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for L-33/ST2. Standards or samples are then added to the microtiter plate wells and L-33/ST2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of L-33/ST2 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for L-33/ST2 are added to each well to "sandwich" the L-33/ST2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain L-33/ST2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The L-33/ST2 concentration in each sample is interpolated from this standard curve.

Intended Uses: This L-33/ST2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human L-33/ST2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for IL-33/ST2 elisa kit

Human ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 314122337
• NCBI GeneID: 90865
• NCBI Accession #: NP_001186570.1
• NCBI GenBank Nucleotide #: NM_001199641.1
• UniProt Accession #: O95760; Q2YEJ5; D3DRI5
• Molecular Weight: 30,759 Da
• NCBI Official Full Name: interleukin-33 isoform 3
• NCBI Official Synonym Full Names: interleukin 33
• NCBI Official Symbol: IL33
• NCBI Official Synonym Symbols: DVS27; IL1F11; NF-HEV; NFEHEV; C9orf26; DKFZp586H0523; RP11-575C20.2
• NCBI Protein Information: interleukin-33; IL-33; IL-1F11; OTTHUMP00000021041; DVS27-related protein; interleukin-1 family member 11; nuclear factor for high endothelial venules; nuclear factor from high endothelial venules
• UniProt Protein Name: Interleukin-33
• UniProt Gene Name: IL33
• UniProt Synonym Gene Names: C9orf26; IL1F11; NFHEV
• UniProt Entry Name: IL33_HUMAN