Interleukin-8 ELISA Kit | IL-8 elisa kit

Interleukin-8 (IL-8)

Cat. Num. AAA77852

• Gene Names: CXCL8; IL8; NAF; GCP1; LECT; LUCT; NAP1; GCP-1; LYNAP; MDNCF; MONAP; NAP-1
• Synonyms: Interleukin-8; N/A; Interleukin-8 (IL-8); IL-8 elisa kit

• Specificity: This sandwich ELISA recognizes both natural and recombinant human IL-8. This kit exhibits no detectable cross-reactivity with human IL-1?, EGF, MCAF, RANTES, and SAA.
• Sequence Length: 99
• Samples: Cell Culture Supernatant, serum, plasma, and other biological fluids
• Assay Type: Sandwich
• Sensitivity: The minimum detectable dose of IL-8 was determined by adding two standard deviations to the mean optical density value of the 20 zero-standard replicates and calculating the corresponding concentration from the standard curve. The minimum detectable dose using a standard curve generated with Calibrator Diluent I is 10 pg/mL and using Calibrator Diluent II is 4 pg/mL.

• Intra-assay Precision: To determine within-run precision, three different samples of known concentration were assayed by using 20 replicates in 1 assay.
• Inter-assay Precision: To determine between-run precision, three different samples of known concentration were assayed by using replicates on 20 different assays.
• Intended Uses: This Human IL-8 ELISA kit is to be used for the in vitro quantitative determination of human interleukin-8 (IL-8) concentrations in cell culture supernatant, serum, plasma, and other biological fluids. This kit is intended for LABORATORY RESEARCH USE ONLY and is not for use in therapeutic procedures.

Standard Curve (Sample)

Related Product Information for IL-8 elisa kit

Principle of the assay: This IL-8 enzyme-linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for IL-8. Standards or samples are then added to the appropriate microtiter plate wells and incubated. IL-8, if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove unbound IL-8 and other components of the sample. In order to quantify the amount of IL-8 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated monoclonal antibody specific for IL-8 is added to each well to "sandwich" the IL-8 immobilized during the first incubation. The microtiter plate then undergoes a second incubation. The wells are thoroughly washed to remove all unbound HRP-conjugated antibodies and a TMB (3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-8 and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450nm +/- 2nm. In order to measure the concentration of IL-8 in the samples, this kit contains two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant/ urine testing). According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D.) versus IL-8 concentration (pg/mL). The concentration of IL-8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for IL-8 elisa kit

ELISA Kits for Human Cytokines and Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 10834978
• NCBI GeneID: 3576
• NCBI Accession #: NP_000575.1
• NCBI GenBank Nucleotide #: NM_000584.3
• Molecular Weight: 11,338 Da
• NCBI Official Full Name: interleukin-8
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 8
• NCBI Official Symbol: CXCL8
• NCBI Official Synonym Symbols: IL8; NAF; GCP1; LECT; LUCT; NAP1; GCP-1; LYNAP; MDNCF; MONAP; NAP-1
• NCBI Protein Information: interleukin-8; T-cell chemotactic factor; alveolar macrophage chemotactic factor I; beta endothelial cell-derived neutrophil activating peptide; beta-thromboglobulin-like protein; emoctakin; granulocyte chemotactic protein 1; interleukin 8; lung giant cell carcinoma-derived chemotactic protein; lymphocyte derived neutrophil activating peptide; monocyte-derived neutrophil chemotactic factor; monocyte-derived neutrophil-activating peptide; neutrophil-activating peptide 1; small inducible cytokine subfamily B, member 8; tumor necrosis factor-induced gene 1
• UniProt Protein Name: Interleukin-8
• UniProt Gene Name: CXCL8
• UniProt Entry Name: IL8_HUMAN