Interleukin 10 Active Protein | IL10 active protein

Active Interleukin 10 (IL10)

Cat. Num. AAA150055

• Gene Names: Il10; CSIF; Il-10
• Applications: Activity Assay, Cell Culture
• Purity: >97%
• Synonyms: Interleukin 10; N/A; Active Interleukin 10 (IL10); CSIF; IL10A; TGIF; Cytokine Synthesis Inhibitory Factor; IL10 active protein

• Purity/Purification: >97%
• Form/Format: Freeze-dried powder; 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Sequence Positions: Ser19~Ser178
• Sequence Length: 178

• Applicable Applications for IL10 active protein: Activity Assay, Cell Culture
• Species: Mouse
• Tag: N-terminal His Tag
• Isoelectric Point: 8.2
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Avoid repeated freeze/thaw cycles.; Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Bioactivity

(Figure. The binding activity of IL-10 with IL10Ra.Interleukin 10 (IL-10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. IL-10 is a cytokine with multiple, pleiotropic, effects in immunoregulation and inflammation. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Besides, Interleukin 10 Receptor Alpha (IL10Ra) has been identified as an interactor of IL-10, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse IL-10 and recombinant mouse IL10Ra. Briefly, IL-10 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100L were then transferred to IL10Ra-coated microtiter wells and incubated for 2h at 37. Wells were washed with PBST and incubated for 1h with anti-IL-10 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL-10 and IL10Ra was shown in Figure 1, and this effect was in a dose dependent manner.)

Gene Sequencing

(Figure. Gene Sequencing (Extract))

WB (Western Blot)

(Figure. Western Blot)

SDS-PAGE

(Figure. SDS-PAGE)

Product Categories/Family for IL10 active protein

Cytokine; Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 6754318
• NCBI GeneID: 16153
• NCBI Accession #: NP_034678.1
• NCBI GenBank Nucleotide #: NP_034678.1
• UniProt Accession #: P18893
• Molecular Weight: Predicted Molecular Mass: 20.4kDa; Accurate Molecular Mass: 22kDa
• NCBI Official Full Name: interleukin-10
• NCBI Official Synonym Full Names: interleukin 10
• NCBI Official Symbol: Il10
• NCBI Official Synonym Symbols: CSIF; Il-10
• NCBI Protein Information: interleukin-10
• UniProt Protein Name: Interleukin-10
• UniProt Gene Name: Il10
• UniProt Synonym Gene Names: Il-10; IL-10; CSIF
• UniProt Entry Name: IL10_MOUSE