ELISA
(Biotinylated Human IL-12 Protein ELISA DataImmobilized Anti-IL-12 Antibody, hFc Tag at 0.5?g/ml (100?l/well) on the plate. Dose response curve for Biotinylated Human IL-12, His Tag with the EC50 of 9.3ng/ml determined by ELISA.)
IL-12 recombinant protein
Biotinylated Human IL-12 Protein Recombinant
Gene Names
IL12B; CLMF; NKSF; CLMF2; NKSF2; IL-12B
Purity
>95%, as determined by SDS-PAGE and HPLC
Synonyms
IL-12; N/A; Biotinylated Human IL-12 Protein Recombinant; IL-12 recombinant protein
Host
HEK293 cells (Note: Host protein expression may be lot-specific and can be subject to change during production, including bulk orders.)
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC
Form/Format
Recombinant Human IL-12 was lyophilized from 0.2 um filtered PBS solution pH7.4
Source
DNA sequence encoding Human IL-12 including a C-His-Avi tag was expressed in HEK293 Cells.The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Molecular Function
Cytokine
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at-20 degree C.
ELISA
(Biotinylated Human IL-12 Protein ELISA DataImmobilized Anti-IL-12 Antibody, hFc Tag at 0.5?g/ml (100?l/well) on the plate. Dose response curve for Biotinylated Human IL-12, His Tag with the EC50 of 9.3ng/ml determined by ELISA.)
ELISA
(Biotinylated Human IL-12 Protein ELISA DataImmobilized Anti-IL-12 Antibody, hFc Tag at 0.5?g/ml (100?l/well) on the plate. Dose response curve for Biotinylated Human IL-12, His Tag with the EC50 of 9.3ng/ml determined by ELISA.)
Application Data
(Biotinylated Human IL-12 Protein SEC-HPLCThe purity of Bitinylated Human IL-12 is greater than 95% as determined by SEC-HPLC.)
Application Data
(Biotinylated Human IL-12 Protein SEC-HPLCThe purity of Bitinylated Human IL-12 is greater than 95% as determined by SEC-HPLC.)
Tris-Bis-PAGE
(Biotinylated Human IL-12 Protein Tris-Bis PAGEBiotinylated Human IL-12 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.)
Tris-Bis-PAGE
(Biotinylated Human IL-12 Protein Tris-Bis PAGEBiotinylated Human IL-12 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.)
Product Categories/Family for IL-12 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
328
NCBI Official Full Name
interleukin-12 subunit beta
NCBI Official Synonym Full Names
interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
NCBI Official Symbol
IL12B
NCBI Official Synonym Symbols
CLMF; NKSF; CLMF2; NKSF2; IL-12B
NCBI Protein Information
interleukin-12 subunit beta; CLMF p40; IL-12 subunit p40; IL12, subunit p40; interleukin 12, p40; interleukin-12 beta chain; NK cell stimulatory factor chain 2; cytotoxic lymphocyte maturation factor 40 kDa subunit; natural killer cell stimulatory factor,
UniProt Protein Name
Interleukin-12 subunit beta
UniProt Gene Name
IL12B
UniProt Synonym Gene Names
NKSF2; IL-12B; CLMF p40; NKSF2
UniProt Entry Name
IL12B_HUMAN
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Product Notes
The IL-12 il12b (Catalog #AAA76190) is a Recombinant Protein produced from HEK293 cells (Note: Host protein expression may be lot-specific and can be subject to change during production, including bulk orders.) and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "IL-12, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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