Interleukin-13 Recombinant Protein | IL-13 recombinant protein
Human Interleukin-13 Recombinant
Purity
>95%, as determined by SDS-PAGE and HPLC
Synonyms
Interleukin-13; N/A; Human Interleukin-13 Recombinant; il13; il-13; nc30; interleukin-13; IL-13 recombinant protein
Host
E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC
Form/Format
Recombinant Interleukin-13 was lyophilized from a 0.2um filtered concentrated (1mg/ml) solution in PBS, pH 7.2.
Sequence
MALLLTTVIA LTCLGGFASP GPVPPSTALR ELIEELVNIT QNQKAPLCNG SMVWSINLTA GMYCAALESL INVSGCSAIE KTQRMLSGFC PHKVSAGQFS SLHVRDTKIE VAQFVKDLLL HLKKLFREGR FN
Sequence Length
146
Domain
4 helix cytokine core
Host Note
Optimized DNA sequence encoding Human Interleukin-13 mature chain was expressed in Escherichia Coli.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Molecular Weight Note
Native human Interleukin-13, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 13 kDa.
Recombinant IL-13 is a disulfide-linked monomer protein consisting of 115 amino acid residue subunits,and migrates as an approximately 13 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Recombinant IL-13 is a disulfide-linked monomer protein consisting of 115 amino acid residue subunits,and migrates as an approximately 13 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
Related Product Information for IL-13 recombinant protein
Mouse IL13 is produced primarily by activated Th2 cells. Human IL13 is expressed in activated T- helper cells (resembling Th0, Th1, Th2), and T-cells expressing CD8. The alpha chain of the IL13 receptor has weak binding activity for IL13. High affinity receptors are formed when the receptor alpha chains of IL13 and IL4 receptors are coexpressed. The receptors for IL13 and IL4 share a component,a common gamma subunit that is found also in the IL2 receptor.Different IL13 receptor structures have been shown to exist on various cell types and the IL13 receptor may share more than one component with IL4 receptor. IL13 down-modulates macrophage activity, reducing the production of pro-inflammatory cytokines and chemokines in response to IFN-gamma or bacterial lypopolysaccharides. IL13 enhances the production of the IL1 receptor antagonist IL1ra. IL13 also decreases the production of nitric oxide by activated macrophages, leading to a decrease in parasiticidal activity. IL13 induces differentiation of human monocytes, enhances survival time in culture, and also induces differentiation and proliferation and Isotype switching in B-cells.
Product Categories/Family for IL-13 recombinant protein
References
• IL-13-induced oxidative stress via microglial nadph oxidase contributes to death of hippocampal neurons in vivo .J. Immunol., Oct 2009; 183: 4666 - 4674.
• involvement of IL-13 in tobacco smoke induced changes in the structure and function of rat intrapulmonary airways .Am. J. Respir. Cell Mol. Biol., Sep 2009; 10.1165/rcmb.2009-0117 degree C.
• disruption of th2 immunity results in a gender-specific expansion of IL-13 producing accessory nk cells during helminth infection .J. Immunol., Sep 2009; 183: 3906 - 3914.
• distinct structural requirements for interleukin-4 (il-4) and IL-13 binding to the shared IL-13 receptor facilitate cellular tuning of cytokine responsiveness .J. Biol. Chem., Sep 2009; 284: 24289 - 24296.
• IL-13 receptor 2 regulates the immune and functional response to nippostrongylus brasiliensis infection .J. Immunol., Aug 2009; 183: 1934 - 1939.
• involvement of IL-13 in tobacco smoke induced changes in the structure and function of rat intrapulmonary airways .Am. J. Respir. Cell Mol. Biol., Sep 2009; 10.1165/rcmb.2009-0117 degree C.
• disruption of th2 immunity results in a gender-specific expansion of IL-13 producing accessory nk cells during helminth infection .J. Immunol., Sep 2009; 183: 3906 - 3914.
• distinct structural requirements for interleukin-4 (il-4) and IL-13 binding to the shared IL-13 receptor facilitate cellular tuning of cytokine responsiveness .J. Biol. Chem., Sep 2009; 284: 24289 - 24296.
• IL-13 receptor 2 regulates the immune and functional response to nippostrongylus brasiliensis infection .J. Immunol., Aug 2009; 183: 1934 - 1939.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15,816 Da
NCBI Official Full Name
interleukin-13
UniProt Protein Name
Interleukin-13
UniProt Gene Name
IL13
UniProt Synonym Gene Names
NC30; IL-13
UniProt Entry Name
IL13_HUMAN
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Product Notes
The IL-13 il13 (Catalog #AAA76093) is a Recombinant Protein produced from E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders) and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: MALLLTTVIA LTCLGGFASP GPVPPSTALR ELIEELVNIT QNQKAPLCNG SMVWSINLTA GMYCAALESL INVSGCSAIE KTQRMLSGFC PHKVSAGQFS SLHVRDTKIE VAQFVKDLLL HLKKLFREGR FN. It is sometimes possible for the material contained within the vial of "Interleukin-13, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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