Standard Curve (Sample)
Human IL-16 ELISA Kit | IL16 elisa kit
Human IL-16 PicoKine ELISA Kit
Gene Names
IL16; LCF; NIL16; PRIL16; prIL-16
Reactivity
Human
Synonyms
IL-16; N/A; Human IL-16 PicoKine ELISA Kit; Pro-interleukin-16; interleukin 16; FLJ16806; FLJ42735; FLJ44234; HGNC:5980; HsT19289; IL16; IL16_HUMAN; Interleukin 16; Interleukin 16 precursor; Interleukin-16; KIAA4048; LCF; Lymphocyte chemoattractant factor; mKIAA4048; Neuronal interleukin 16; NIL16; OTTHUMP00000185571; OTTHUMP00000190849; OTTHUMP00000190850; PrIL16; Pro interleukin 16; Prointerleukin 16; IL16 elisa kit
Reactivity
Human
Specificity
Natural and recombinant human IL-16
Sequence Length
1332
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
31.2pg/ml-2000pg/ml
Sensitivity
< 1pg/ml
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for IL16 elisa kit
Principle of the assay: human IL-16 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-16 has been precoated onto 96-well plates. Standards(E.coli, P2-S130) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-16 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-16 amount of sample captured in plate.
Background: Interleukin 16 (IL-16) is a cytokine that released by a variety of cells (including lymphocytes and some epithelial cells) that has been characterized as a chemoattractant for certain immune cells expressing the cell surface molecule CD4. By Southern blot analysis and PCR using a human/rodent somatic cell hybrid mapping panel, The human IL16 is encoded by a single-copy gene on chromosome 15. Using a combination of STS-content mapping, radiation-hybrid mapping, and genetic mapping, it was refined the assignment to 15q26.1. The mouse Il16 gene was mapped to chromosome 7 in a region showing homology of synteny to human 15q26.1. IL-16 was originally described as a factor that could attract activated T cells in humans, it was previously called lymphocyte chemoattractant factor (LCF), and the augmentation of IL16stimulation by CCR5 plays a role in regulation of Th1 cell recruitment and activation at sites of inflammation.
Background: Interleukin 16 (IL-16) is a cytokine that released by a variety of cells (including lymphocytes and some epithelial cells) that has been characterized as a chemoattractant for certain immune cells expressing the cell surface molecule CD4. By Southern blot analysis and PCR using a human/rodent somatic cell hybrid mapping panel, The human IL16 is encoded by a single-copy gene on chromosome 15. Using a combination of STS-content mapping, radiation-hybrid mapping, and genetic mapping, it was refined the assignment to 15q26.1. The mouse Il16 gene was mapped to chromosome 7 in a region showing homology of synteny to human 15q26.1. IL-16 was originally described as a factor that could attract activated T cells in humans, it was previously called lymphocyte chemoattractant factor (LCF), and the augmentation of IL16stimulation by CCR5 plays a role in regulation of Th1 cell recruitment and activation at sites of inflammation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
66,646 Da
NCBI Official Full Name
Pro-interleukin-16
NCBI Official Synonym Full Names
interleukin 16
NCBI Official Symbol
IL16
NCBI Official Synonym Symbols
LCF; NIL16; PRIL16; prIL-16
NCBI Protein Information
pro-interleukin-16; prointerleukin 16; neuronal interleukin 16; lymphocyte chemoattractant factor
UniProt Protein Name
Pro-interleukin-16
UniProt Gene Name
IL16
UniProt Synonym Gene Names
IL-16; LCF
UniProt Entry Name
IL16_HUMAN
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Product Notes
The Human IL16 il16 (Catalog #AAA45527) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA45527 ELISA Kit recognizes Human IL16. It is sometimes possible for the material contained within the vial of "IL-16, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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