IL-1 receptor type II (CD121b) Recombinant Protein | IL1R2 recombinant protein
Mouse IL-1 receptor type II Recombinant (CD121b)
Applications
ELISA
Purity
>97%, as determined by SDS-PAGE and HPLC
Synonyms
IL-1 receptor type II (CD121b); N/A; Mouse IL-1 receptor type II Recombinant (CD121b); Mouse IL-1 receptor type II Recombinant; CD121B; D2S1473; IL-1R1; IL1R2; IL1RB; P80; IL1R2 recombinant protein
Purity/Purification
>97%, as determined by SDS-PAGE and HPLC
Form/Format
Recombinant Mouse IL-1 receptor II (CD121b) is lyophilized from 0.2 um filtered PBS solution, pH7.2 , 5% Trehalose, 0.01% tween80.
Applicable Applications for IL1R2 recombinant protein
ELISA
Source
Optimized DNA sequence encoding Mouse extracellular domain of mouse IL-1 receptor type II including a C-terminal His tag was expressed in
HEK293 cells.
Biological Activity
Measured in an ELISA assay. The ability of mouse IL1R2 (10ug/ml) to bind biotinylated human IL1B was determined to be 0.5 ug/ml
Endotoxin
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug(1EU/ug).
Endotoxin level was found to be less than 0.1 ng/ug(1EU/ug).
Preparation and Storage
Recombinant Mouse IL1R2 can be stored in working aliquots at 2 degree C - 8 degree C for 1 month, or at -20 degree C to -70 degree C for long term (12 months).
Avoid repeated freeze/thaw cycles.
Avoid repeated freeze/thaw cycles.
Product Categories/Family for IL1R2 recombinant protein
References
• pellino 2 is critical for toll-like receptor/interleukin-1 receptor (tlr/IL-1R) -mediated post-transcriptional control .J. Biol. Chem., Jul 2012; 287: 25686 - 25695.
• role of toll interleukin-1 receptor (IL-1R) 8, a negative regulator of IL-1R/toll-like receptor signaling, in resistance to acute pseudomonas aeruginosa lung infection .Infect. Immun., Jan 2012; 80: 100 - 109.
• IL-1R–associated kinase-1 mediates protein kinase c-induced il-1β production in monocytes .J. Immunol., Sep 2011; 187: 2632 - 2645.
• effect of mitomycin c on IL-1R expression, il-1–related hepatocyte growth factor secretion and corneal epithelial cell migration .Invest. Ophthalmol. Vis. Sci., Mar 2010; 51: 1389 - 1396.
• mycobacterium bovis bacillus calmette-guérin vaccination mobilizes innate myeloid-derived suppressor cells restraining in vivo t cell priming via IL-1R–dependent nitric oxide production .J. Immunol., Feb 2010; 184: 2038 - 2047.
• role of toll interleukin-1 receptor (IL-1R) 8, a negative regulator of IL-1R/toll-like receptor signaling, in resistance to acute pseudomonas aeruginosa lung infection .Infect. Immun., Jan 2012; 80: 100 - 109.
• IL-1R–associated kinase-1 mediates protein kinase c-induced il-1β production in monocytes .J. Immunol., Sep 2011; 187: 2632 - 2645.
• effect of mitomycin c on IL-1R expression, il-1–related hepatocyte growth factor secretion and corneal epithelial cell migration .Invest. Ophthalmol. Vis. Sci., Mar 2010; 51: 1389 - 1396.
• mycobacterium bovis bacillus calmette-guérin vaccination mobilizes innate myeloid-derived suppressor cells restraining in vivo t cell priming via IL-1R–dependent nitric oxide production .J. Immunol., Feb 2010; 184: 2038 - 2047.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Recombinant mouse IL-1 receptor type II (CD121b) is a protein consisting of 350 amino acid residue subunits, due to glycosylation migrates as an approximately 90 kDa protein on SDS-PAGE.
NCBI Official Full Name
interleukin-1 receptor type 2
UniProt Protein Name
Interleukin-1 receptor type 2
UniProt Gene Name
Il1r2
UniProt Synonym Gene Names
Il-1r2; Il1rb; IL-1R-2; IL-1RT-2; IL-1RT2; IL-1R-beta; mIL-1R2; mIL-1RII; sIL-1R2; sIL-1RII
UniProt Entry Name
IL1R2_MOUSE
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Product Notes
The IL1R2 il1r2 (Catalog #AAA76109) is a Recombinant Protein and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's IL-1 receptor type II (CD121b) can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the IL1R2 il1r2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-1 receptor type II (CD121b), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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