Standard Curve (Sample)
Mouse IL-1RA ELISA Kit | IL1RN elisa kit
Mouse IL-1RA ELISA Kit
Gene Names
Il1rn; IL-1ra; F630041P17Rik
Reactivity
Mouse
Applications
ELISA
Synonyms
IL-1RA; Mouse IL-1RA ELISA Kit; IL1F3; IL1RA; Interleukin-1 receptor antagonist protein; IL-1RN; IL-1ra; IRAP; ICIL-1RA; IL1 inhibitor; Anakinra; IL1RN elisa kit
Reactivity
Mouse
Specificity
The Mouse IL-1RA ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse IL-1RA proteins within the range of 156 pg/ml - 10,000 pg/ml.
Sequence Length
178
Samples
Culture Supernatants, Serum, Plasma
Sensitivity
< 80 pg/ml
Applicable Applications for IL1RN elisa kit
Sandwich ELISA (SE)
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for IL1RN elisa kit
Background: The interleukin-1 receptor antagonist (IL-1RA) is a protein that in humans is encoded by the IL1RN gene. The human IL1RN gene was localized to the long arm of chromosome 2 at band 2q14. by fluorescence in situ hybridization. IL-1RA is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses.
Principle of the Assay: The Mouse IL-1RA ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-1RA in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse IL-1RA coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1RA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse IL-1RA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1RA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Principle of the Assay: The Mouse IL-1RA ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-1RA in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse IL-1RA coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1RA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse IL-1RA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1RA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,996 Da
NCBI Official Full Name
interleukin-1 receptor antagonist protein isoform 2
NCBI Official Synonym Full Names
interleukin 1 receptor antagonist
NCBI Official Symbol
Il1rn
NCBI Official Synonym Symbols
IL-1ra; F630041P17Rik
NCBI Protein Information
interleukin-1 receptor antagonist protein; IRAP; IL-1RN; IL1 inhibitor
UniProt Protein Name
Interleukin-1 receptor antagonist protein
UniProt Gene Name
Il1rn
UniProt Synonym Gene Names
Il-1ra; IL-1RN; IL-1ra; IRAP
UniProt Entry Name
IL1RA_MOUSE
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Product Notes
The Mouse IL1RN il1rn (Catalog #AAA17868) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA17868 ELISA Kit recognizes Mouse IL1RN. AAA Biotech's IL-1RA can be used in a range of immunoassay formats including, but not limited to, Sandwich ELISA (SE). Researchers should empirically determine the suitability of the IL1RN il1rn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-1RA, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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